Functional recovery of biofilm bacterial communities after copper exposure

M.E.Y. Boivin, B. Massieux, A.M. Breure, G.D. Greve, M. Rutgers, W. Admiraal

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    Potential of bacterial Communities in biofilms to recover after copper exposure was investigated. Biofilms grown outdoor in shallow water on glass dishes were exposed ill the laboratory to 0.6, 2.1, 6.8 mu mol/l copper amended surface water and a reference and subsequently to un-amended surface water. Transitions of bacterial communities were characterised with denaturing gradient gel electrophoresis (DGGE) and community-level physiological profiles (CLPP). Exposure to 6.8 mu mol/l copper provoked distinct changes in DGGE profiles of bacterial consortia, which did not reverse upon copper depuration. Exposure to 2.1 and 6.8 mu mol/l copper was found to induce marked changes ill CLPP of bacterial communities that proved to be reversible during copper depuration. Furthermore, copper exposure induced the development of copper-tolerance, which was partially lost during depuration. It is concluded that bacterial communities exposed to copper contaminated water for a period of 26 days are capable to restore their metabolic attributes after introduction of unpolluted water in aquaria for 28 days. [KEYWORDS: pollution-induced community tolerance (PICT) ; community-level physiological profiling (CLPP) ; denaturing gradient gel electrophoresis (DGGE) ; bacterial communities ; recovery]
    Originele taal-2Engels
    Pagina's (van-tot)239-246
    TijdschriftEnvironmental Pollution
    Volume140
    Nummer van het tijdschrift2
    DOI's
    StatusGepubliceerd - 2006

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