Generation of BAC Transgenic Epithelial Organoids

G. Schwank, A. Andersson-Rolf, B.K. Koo, N. Sasaki, H. Clevers

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Under previously developed culture conditions, mouse and human intestinal epithelia can be cultured and expanded over long periods. These so-called organoids recapitulate the three-dimensional architecture of the gut epithelium, and consist of all major intestinal cell types. One key advantage of these ex vivo cultures is their accessibility to live imaging. So far the establishment of transgenic fluorescent reporter organoids has required the generation of transgenic mice, a laborious and time-consuming process, which cannot be extended to human cultures. Here we present a transfection protocol that enables the generation of recombinant mouse and human reporter organoids using BAC (bacterial artificial chromosome) technology.
Originele taal-2Engels
Pagina's (van-tot)76871
TijdschriftPLoS One
Volume8
Nummer van het tijdschrift10
DOI's
StatusGepubliceerd - 2013

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