Genome editing by natural and engineered CRISPR-associated nucleases

Wen Y Wu, Joyce H G Lebbink, Roland Kanaar, Niels Geijsen, John van der Oost

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.

Originele taal-2Engels
Pagina's (van-tot)642-651
Aantal pagina's10
TijdschriftNature Chemical Biology
Volume14
Nummer van het tijdschrift7
DOI's
StatusGepubliceerd - jul 2018

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