Genome editing by natural and engineered CRISPR-associated nucleases

TY - JOUR

T1 - Genome editing by natural and engineered CRISPR-associated nucleases

AU - Wu, Wen Y

AU - Lebbink, Joyce H G

AU - Kanaar, Roland

AU - Geijsen, Niels

AU - van der Oost, John

PY - 2018/7

Y1 - 2018/7

N2 - Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.

AB - Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.

U2 - 10.1038/s41589-018-0080-x

DO - 10.1038/s41589-018-0080-x

M3 - Article

C2 - 29915237

SN - 1552-4450

VL - 14

SP - 642

EP - 651

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 7

ER -