Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells

Jop Kind, Ludo Pagie, Sandra S de Vries, Leila Nahidiazar, Siddharth S Dey, Magda Bienko, Ye Zhan, Bryan Lajoie, Carolyn A de Graaf, Mario Amendola, Geoffrey Fudenberg, Maxim Imakaev, Leonid A Mirny, Kees Jalink, Job Dekker, Alexander van Oudenaarden, Bas van Steensel

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization. VIDEO ABSTRACT.

Originele taal-2Engels
Pagina's (van-tot)134-47
Aantal pagina's14
TijdschriftCell
Volume163
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 24 sep 2015

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