Heterogeneity in kinesin function

Babu J N Reddy; Suvranta Tripathy; Michael Vershinin; Marvin E Tanenbaum; Jing Xu; Michelle Mattson-Hoss; Karim Arabi; Dail Chapman; Tory Doolin; Changbong Hyeon; Steven P Gross

doi:10.1111/tra.12504

Heterogeneity in kinesin function

Babu J N Reddy, Suvranta Tripathy, Michael Vershinin, Marvin E Tanenbaum, Jing Xu, Michelle Mattson-Hoss, Karim Arabi, Dail Chapman, Tory Doolin, Changbong Hyeon, Steven P Gross

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

12 Citaten (Scopus)

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The kinesin family proteins are often studied as prototypical molecular motors; a deeper understanding of them can illuminate regulation of intracellular transport. It is typically assumed that they function identically. Here we find that this assumption of homogeneous function appears incorrect: variation among motors' velocities in vivo and in vitro is larger than the stochastic variation expected for an ensemble of "identical" motors. When moving on microtubules, slow and fast motors are persistently slow, and fast, respectively. We develop theory that provides quantitative criteria to determine whether the observed single-molecule variation is too large to be generated from an ensemble of identical molecules. To analyze such heterogeneity, we group traces into homogeneous sub-ensembles. Motility studies varying the temperature, pH and glycerol concentration suggest at least 2 distinct functional states that are independently affected by external conditions. We end by investigating the functional ramifications of such heterogeneity through Monte-Carlo multi-motor simulations.

Originele taal-2	Engels
Pagina's (van-tot)	658-671
Aantal pagina's	14
Tijdschrift	Traffic
Volume	18
Nummer van het tijdschrift	10
DOI's	https://doi.org/10.1111/tra.12504
Status	Gepubliceerd - okt. 2017

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10.1111/tra.12504

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AU - Reddy, Babu J N

AU - Tripathy, Suvranta

AU - Vershinin, Michael

AU - Tanenbaum, Marvin E

AU - Xu, Jing

AU - Mattson-Hoss, Michelle

AU - Arabi, Karim

AU - Chapman, Dail

AU - Doolin, Tory

AU - Hyeon, Changbong

AU - Gross, Steven P

PY - 2017/10

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AB - The kinesin family proteins are often studied as prototypical molecular motors; a deeper understanding of them can illuminate regulation of intracellular transport. It is typically assumed that they function identically. Here we find that this assumption of homogeneous function appears incorrect: variation among motors' velocities in vivo and in vitro is larger than the stochastic variation expected for an ensemble of "identical" motors. When moving on microtubules, slow and fast motors are persistently slow, and fast, respectively. We develop theory that provides quantitative criteria to determine whether the observed single-molecule variation is too large to be generated from an ensemble of identical molecules. To analyze such heterogeneity, we group traces into homogeneous sub-ensembles. Motility studies varying the temperature, pH and glycerol concentration suggest at least 2 distinct functional states that are independently affected by external conditions. We end by investigating the functional ramifications of such heterogeneity through Monte-Carlo multi-motor simulations.

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DO - 10.1111/tra.12504

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Heterogeneity in kinesin function

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