Hoxb8-Cre mice: A tool for brain-sparing conditional gene deletion

R. Witschi; T. Johansson; G. Morscher; L. Scheurer; J. Deschamps; H.U. Zeilhofer

doi:10.1002/dvg.20656

Hoxb8-Cre mice: A tool for brain-sparing conditional gene deletion

R. Witschi, T. Johansson, G. Morscher, L. Scheurer, J. Deschamps, H.U. Zeilhofer

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

26 Citaten (Scopus)

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The spinal cord is the first site of temporal and spatial integration of nociceptive signals in the pain pathway. Neuroplastic changes occurring at this site contribute critically to various chronic pain syndromes. Gene targeting in mice has generated important insights into these processes. However, the analysis of constitutive (global) gene-deficient mice is often hampered by confounding effects arising from supraspinal sites. Here, we describe a novel Cre mouse line that expresses the Cre recombinase under the transcriptional control of the Hoxb8 gene. Within the neural axis of these mice, Hoxb8-Cre expression is found in spinal cord neurons and glial cells, and in virtually all neurons of the dorsal root ganglia, but spares the brain apart from a few cells in the spinal trigeminal nucleus. The Hoxb8-Cre mouse line should be a valuable new tool for the in vivo analysis of peripheral and spinal gene functions in pain pathways.

Originele taal-2	Engels
Pagina's (van-tot)	596-602
Tijdschrift	Genesis
Volume	48
Nummer van het tijdschrift	10
DOI's	https://doi.org/10.1002/dvg.20656
Status	Gepubliceerd - 2010

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Hoxb8-Cre mice: A tool for brain-sparing conditional gene deletion

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