HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis

Inger Brandsma; Koichi Sato; Sari E van Rossum-Fikkert; Nicole van Vliet; Esther Sleddens; Marcel Reuter; Hanny Odijk; Nathalie van den Tempel; Dick H W Dekkers; Karel Bezstarosti; Jeroen A A Demmers; Alex Maas; Joyce Lebbink; Claire Wyman; Jeroen Essers; Dik C van Gent; Willy M Baarends; Puck Knipscheer; Roland Kanaar; Alex N Zelensky

doi:10.1016/j.celrep.2019.05.096

HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis

Inger Brandsma, Koichi Sato, Sari E van Rossum-Fikkert, Nicole van Vliet, Esther Sleddens, Marcel Reuter, Hanny Odijk, Nathalie van den Tempel, Dick H W Dekkers, Karel Bezstarosti, Jeroen A A Demmers, Alex Maas, Joyce Lebbink, Claire Wyman, Jeroen Essers, Dik C van Gent, Willy M Baarends, Puck Knipscheer, Roland Kanaar, Alex N Zelensky

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

43 Citaten (Scopus)

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The tumor suppressor BRCA2 is essential for homologous recombination (HR), replication fork stability, and DNA interstrand crosslink repair in vertebrates. We identify HSF2BP, a protein previously described as testis specific and not characterized functionally, as an interactor of BRCA2 in mouse embryonic stem cells, where the 2 proteins form a constitutive complex. HSF2BP is transcribed in all cultured human cancer cell lines tested and elevated in some tumor samples. Inactivation of the mouse Hsf2bp gene results in male infertility due to a severe HR defect during spermatogenesis. The BRCA2-HSF2BP interaction is highly evolutionarily conserved and maps to armadillo repeats in HSF2BP and a 68-amino acid region between the BRC repeats and the DNA binding domain of human BRCA2 (Gly2270-Thr2337) encoded by exons 12 and 13. This region of BRCA2 does not harbor known cancer-associated missense mutations and may be involved in the reproductive rather than the tumor-suppressing function of BRCA2.

Originele taal-2	Engels
Pagina's (van-tot)	3790-3798.e7
Tijdschrift	Cell Reports
Volume	27
Nummer van het tijdschrift	13
DOI's	https://doi.org/10.1016/j.celrep.2019.05.096
Status	Gepubliceerd - 25 jun. 2019

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10.1016/j.celrep.2019.05.096

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Brandsma, I., Sato, K., van Rossum-Fikkert, S. E., van Vliet, N., Sleddens, E., Reuter, M., Odijk, H., van den Tempel, N., Dekkers, D. H. W., Bezstarosti, K., Demmers, J. A. A., Maas, A., Lebbink, J., Wyman, C., Essers, J., van Gent, D. C., Baarends, W. M., Knipscheer, P., Kanaar, R., & Zelensky, A. N. (2019). HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis. Cell Reports, 27(13), 3790-3798.e7. https://doi.org/10.1016/j.celrep.2019.05.096

@article{3dc80fe2041c4658813dcd8bda6507ca,

title = "HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis",

abstract = "The tumor suppressor BRCA2 is essential for homologous recombination (HR), replication fork stability, and DNA interstrand crosslink repair in vertebrates. We identify HSF2BP, a protein previously described as testis specific and not characterized functionally, as an interactor of BRCA2 in mouse embryonic stem cells, where the 2 proteins form a constitutive complex. HSF2BP is transcribed in all cultured human cancer cell lines tested and elevated in some tumor samples. Inactivation of the mouse Hsf2bp gene results in male infertility due to a severe HR defect during spermatogenesis. The BRCA2-HSF2BP interaction is highly evolutionarily conserved and maps to armadillo repeats in HSF2BP and a 68-amino acid region between the BRC repeats and the DNA binding domain of human BRCA2 (Gly2270-Thr2337) encoded by exons 12 and 13. This region of BRCA2 does not harbor known cancer-associated missense mutations and may be involved in the reproductive rather than the tumor-suppressing function of BRCA2.",

author = "Inger Brandsma and Koichi Sato and {van Rossum-Fikkert}, {Sari E} and {van Vliet}, Nicole and Esther Sleddens and Marcel Reuter and Hanny Odijk and {van den Tempel}, Nathalie and Dekkers, {Dick H W} and Karel Bezstarosti and Demmers, {Jeroen A A} and Alex Maas and Joyce Lebbink and Claire Wyman and Jeroen Essers and {van Gent}, {Dik C} and Baarends, {Willy M} and Puck Knipscheer and Roland Kanaar and Zelensky, {Alex N}",

year = "2019",

month = jun,

day = "25",

doi = "10.1016/j.celrep.2019.05.096",

language = "English",

volume = "27",

pages = "3790--3798.e7",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "13",

}

Brandsma, I, Sato, K, van Rossum-Fikkert, SE, van Vliet, N, Sleddens, E, Reuter, M, Odijk, H, van den Tempel, N, Dekkers, DHW, Bezstarosti, K, Demmers, JAA, Maas, A, Lebbink, J, Wyman, C, Essers, J, van Gent, DC, Baarends, WM, Knipscheer, P, Kanaar, R & Zelensky, AN 2019, 'HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis', Cell Reports, vol. 27, nr. 13, blz. 3790-3798.e7. https://doi.org/10.1016/j.celrep.2019.05.096

TY - JOUR

T1 - HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis

AU - Brandsma, Inger

AU - Sato, Koichi

AU - van Rossum-Fikkert, Sari E

AU - van Vliet, Nicole

AU - Sleddens, Esther

AU - Reuter, Marcel

AU - Odijk, Hanny

AU - van den Tempel, Nathalie

AU - Dekkers, Dick H W

AU - Bezstarosti, Karel

AU - Demmers, Jeroen A A

AU - Maas, Alex

AU - Lebbink, Joyce

AU - Wyman, Claire

AU - Essers, Jeroen

AU - van Gent, Dik C

AU - Baarends, Willy M

AU - Knipscheer, Puck

AU - Kanaar, Roland

AU - Zelensky, Alex N

PY - 2019/6/25

Y1 - 2019/6/25

N2 - The tumor suppressor BRCA2 is essential for homologous recombination (HR), replication fork stability, and DNA interstrand crosslink repair in vertebrates. We identify HSF2BP, a protein previously described as testis specific and not characterized functionally, as an interactor of BRCA2 in mouse embryonic stem cells, where the 2 proteins form a constitutive complex. HSF2BP is transcribed in all cultured human cancer cell lines tested and elevated in some tumor samples. Inactivation of the mouse Hsf2bp gene results in male infertility due to a severe HR defect during spermatogenesis. The BRCA2-HSF2BP interaction is highly evolutionarily conserved and maps to armadillo repeats in HSF2BP and a 68-amino acid region between the BRC repeats and the DNA binding domain of human BRCA2 (Gly2270-Thr2337) encoded by exons 12 and 13. This region of BRCA2 does not harbor known cancer-associated missense mutations and may be involved in the reproductive rather than the tumor-suppressing function of BRCA2.

AB - The tumor suppressor BRCA2 is essential for homologous recombination (HR), replication fork stability, and DNA interstrand crosslink repair in vertebrates. We identify HSF2BP, a protein previously described as testis specific and not characterized functionally, as an interactor of BRCA2 in mouse embryonic stem cells, where the 2 proteins form a constitutive complex. HSF2BP is transcribed in all cultured human cancer cell lines tested and elevated in some tumor samples. Inactivation of the mouse Hsf2bp gene results in male infertility due to a severe HR defect during spermatogenesis. The BRCA2-HSF2BP interaction is highly evolutionarily conserved and maps to armadillo repeats in HSF2BP and a 68-amino acid region between the BRC repeats and the DNA binding domain of human BRCA2 (Gly2270-Thr2337) encoded by exons 12 and 13. This region of BRCA2 does not harbor known cancer-associated missense mutations and may be involved in the reproductive rather than the tumor-suppressing function of BRCA2.

U2 - 10.1016/j.celrep.2019.05.096

DO - 10.1016/j.celrep.2019.05.096

M3 - Article

C2 - 31242413

SN - 2211-1247

VL - 27

SP - 3790-3798.e7

JO - Cell Reports

JF - Cell Reports

IS - 13

ER -

HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis

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