Hydrophilins in the filamentous fungus Neosartorya fischeri (Aspergillus fischeri) have protective activity against several types of microbial water stress

TY - JOUR

T1 - Hydrophilins in the filamentous fungus Neosartorya fischeri (Aspergillus fischeri) have protective activity against several types of microbial water stress

AU - van Leeuwen, M R

AU - Wyatt, T T

AU - van Doorn, T M

AU - Lugones, L G

AU - Wösten, H A B

AU - Dijksterhuis, J

N1 - This article is protected by copyright. All rights reserved.

PY - 2015/10/21

Y1 - 2015/10/21

N2 - Hydrophilins are proteins that occur in all domains of life and protect cells and organisms against drought and other stresses. They include most of the Late Embryogenesis Abundant (LEA) proteins and the heat shock protein Hsp12. Here, the role of a predicted LEA-like protein (LeamA) and two Hsp12 proteins (Hsp12A, Hsp12B) of Neosartorya fischeri was studied. This filamentous fungus forms ascospores that belong to the most stress-resistant eukaryotic cells described to date. Heterologous expression of LeamA, Hsp12A, and Hsp12B resulted in increased tolerance against salt and osmotic stress in Escherichia coli. These proteins were also shown to protect lactate dehydrogenase against dry heat and freeze-thaw cycles in vitro. Deletion of leamA caused diminished viability of sexual ascospores after drought and heat. This is the first report on functionality of Hsp 12 and putative LeamA proteins derived from filamentous fungi, and their possible role in N. fischeri ascospore resistance against desiccation, high temperature and osmotic stress is discussed.

AB - Hydrophilins are proteins that occur in all domains of life and protect cells and organisms against drought and other stresses. They include most of the Late Embryogenesis Abundant (LEA) proteins and the heat shock protein Hsp12. Here, the role of a predicted LEA-like protein (LeamA) and two Hsp12 proteins (Hsp12A, Hsp12B) of Neosartorya fischeri was studied. This filamentous fungus forms ascospores that belong to the most stress-resistant eukaryotic cells described to date. Heterologous expression of LeamA, Hsp12A, and Hsp12B resulted in increased tolerance against salt and osmotic stress in Escherichia coli. These proteins were also shown to protect lactate dehydrogenase against dry heat and freeze-thaw cycles in vitro. Deletion of leamA caused diminished viability of sexual ascospores after drought and heat. This is the first report on functionality of Hsp 12 and putative LeamA proteins derived from filamentous fungi, and their possible role in N. fischeri ascospore resistance against desiccation, high temperature and osmotic stress is discussed.

U2 - 10.1111/1758-2229.12349

DO - 10.1111/1758-2229.12349

M3 - Article

C2 - 26487515

SN - 1758-2229

VL - 8

SP - 45

EP - 52

JO - Environmental Microbiology Reports

JF - Environmental Microbiology Reports

IS - 1

ER -