Identification and regulation of a molecular module for bleb-based cell motility

M. Goudarzi; T.U. Banisch; M.B. Mobin; N. Maghelli; K. Tarbashevich; I. Strate; J. ter Berg; H. Blaser; S. Bandemer; E. Paluch; J. Bakkers; I.M. Tolic-Norrelykke; E. Raz

doi:10.1016/j.devcel.2012.05.007

Identification and regulation of a molecular module for bleb-based cell motility

M. Goudarzi, T.U. Banisch, M.B. Mobin, N. Maghelli, K. Tarbashevich, I. Strate, J. ter Berg, H. Blaser, S. Bandemer, E. Paluch, J. Bakkers, I.M. Tolic-Norrelykke, E. Raz

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

53 Citaten (Scopus)

Samenvatting

Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restoring motility. We have defined myosin contractility, cell adhesion, and cortex properties as factors whose proper regulation is sufficient for restoring cell migration of this cell type. Tight control over the level of these cellular features, achieved through a balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment.

Originele taal-2	Engels
Pagina's (van-tot)	210-218
Tijdschrift	Developmental Cell
Volume	23
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1016/j.devcel.2012.05.007
Status	Gepubliceerd - 2012

Toegang tot document

10.1016/j.devcel.2012.05.007

Citeer dit

@article{c8565c881d8d43bf80bb1f98624c1367,

title = "Identification and regulation of a molecular module for bleb-based cell motility",

abstract = "Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restoring motility. We have defined myosin contractility, cell adhesion, and cortex properties as factors whose proper regulation is sufficient for restoring cell migration of this cell type. Tight control over the level of these cellular features, achieved through a balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment.",

author = "M. Goudarzi and T.U. Banisch and M.B. Mobin and N. Maghelli and K. Tarbashevich and I. Strate and {ter Berg}, J. and H. Blaser and S. Bandemer and E. Paluch and J. Bakkers and I.M. Tolic-Norrelykke and E. Raz",

note = "Reporting year: 2012",

year = "2012",

doi = "10.1016/j.devcel.2012.05.007",

language = "English",

volume = "23",

pages = "210--218",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Identification and regulation of a molecular module for bleb-based cell motility

AU - Goudarzi, M.

AU - Banisch, T.U.

AU - Mobin, M.B.

AU - Maghelli, N.

AU - Tarbashevich, K.

AU - Strate, I.

AU - ter Berg, J.

AU - Blaser, H.

AU - Bandemer, S.

AU - Paluch, E.

AU - Bakkers, J.

AU - Tolic-Norrelykke, I.M.

AU - Raz, E.

N1 - Reporting year: 2012

PY - 2012

Y1 - 2012

N2 - Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restoring motility. We have defined myosin contractility, cell adhesion, and cortex properties as factors whose proper regulation is sufficient for restoring cell migration of this cell type. Tight control over the level of these cellular features, achieved through a balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment.

AB - Single-cell migration is a key process in development, homeostasis, and disease. Nevertheless, the control over basic cellular mechanisms directing cells into motile behavior in vivo is largely unknown. Here, we report on the identification of a minimal set of parameters the regulation of which confers proper morphology and cell motility. Zebrafish primordial germ cells rendered immotile by knockdown of Dead end, a negative regulator of miRNA function, were used as a platform for identifying processes restoring motility. We have defined myosin contractility, cell adhesion, and cortex properties as factors whose proper regulation is sufficient for restoring cell migration of this cell type. Tight control over the level of these cellular features, achieved through a balance between miRNA-430 function and the action of the RNA-binding protein Dead end, effectively transforms immotile primordial germ cells into polarized cells that actively migrate relative to cells in their environment.

U2 - 10.1016/j.devcel.2012.05.007

DO - 10.1016/j.devcel.2012.05.007

M3 - Article

SN - 1534-5807

VL - 23

SP - 210

EP - 218

JO - Developmental Cell

JF - Developmental Cell

IS - 1

ER -

Identification and regulation of a molecular module for bleb-based cell motility

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit