Identification of an l-Arabitol Transporter from Aspergillus niger.

TY - JOUR

T1 - Identification of an l-Arabitol Transporter from Aspergillus niger.

AU - Meng, Jiali

AU - Mäkelä, Miia R

AU - de Vries, Ronald P

PY - 2023/1/17

Y1 - 2023/1/17

N2 - l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in Aspergillus niger. Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered A. niger mutant by the deletion of latA, indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.

AB - l-arabitol is an intermediate of the pentose catabolic pathway in fungi but can also be used as a carbon source by many fungi, suggesting the presence of transporters for this polyol. In this study, an l-arabitol transporter, LatA, was identified in Aspergillus niger. Growth and expression profiles as well as sugar consumption analysis indicated that LatA only imports l-arabitol and is regulated by the arabinanolytic transcriptional activator AraR. Moreover, l-arabitol production from wheat bran was increased in a metabolically engineered A. niger mutant by the deletion of latA, indicating its potential for improving l-arabitol-producing cell factories. Phylogenetic analysis showed that homologs of LatA are widely conserved in fungi.

KW - Aspergillus niger/metabolism

KW - Phylogeny

KW - Sugar Alcohols/metabolism

U2 - 10.3390/biom13020188

DO - 10.3390/biom13020188

M3 - Article

C2 - 36830558

SN - 2218-273X

VL - 13

JO - Biomolecules

JF - Biomolecules

IS - 2

ER -