Imaging Translation Dynamics of Single mRNA Molecules in Live Cells

Suzan Ruijtenberg, Tim A Hoek, Xiaowei Yan, Marvin E Tanenbaum

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

mRNA translation is a key step in decoding the genetic information stored in DNA. Regulation of translation efficiency contributes to gene expression control and is therefore important for cell fate and function. Here, we describe a recently developed microscopy-based method that allows for visualization of translation of single mRNAs in live cells. The ability to measure translation dynamics of single mRNAs will enable a better understanding of spatiotemporal control of translation, and will provide unique insights into translational heterogeneity of different mRNA molecules in single cells.

Originele taal-2Engels
Pagina's (van-tot)385-404
Aantal pagina's20
TijdschriftMethods in Molecular Biology
Volume1649
DOI's
StatusGepubliceerd - 2018

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