Improved genetic manipulation of human embryonic stem cells.

S.R. Braam; C. Denning; S. van den Brink; P. Kats; R. Hochstenbach; R. Passier; C.L. Mummery

doi:10.1038/nmeth.1200

Improved genetic manipulation of human embryonic stem cells.

S.R. Braam, C. Denning, S. van den Brink, P. Kats, R. Hochstenbach, R. Passier, C.L. Mummery

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

87 Citaten (Scopus)

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Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple independent hESC lines with different growth requirements to standardized feeder-free culture, and optimized conditions for clonal growth and efficient gene transfer without loss of pluripotency. Stably transfected lines retained differentiation potential, and most lines displayed normal karyotypes.

Originele taal-2	Engels
Pagina's (van-tot)	389-392
Tijdschrift	Nature Methods
Volume	5
Nummer van het tijdschrift	5
DOI's	https://doi.org/10.1038/nmeth.1200
Status	Gepubliceerd - 2008

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10.1038/nmeth.1200

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title = "Improved genetic manipulation of human embryonic stem cells.",

abstract = "Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple independent hESC lines with different growth requirements to standardized feeder-free culture, and optimized conditions for clonal growth and efficient gene transfer without loss of pluripotency. Stably transfected lines retained differentiation potential, and most lines displayed normal karyotypes.",

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AU - Denning, C.

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AU - Hochstenbach, R.

AU - Passier, R.

AU - Mummery, C.L.

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AB - Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple independent hESC lines with different growth requirements to standardized feeder-free culture, and optimized conditions for clonal growth and efficient gene transfer without loss of pluripotency. Stably transfected lines retained differentiation potential, and most lines displayed normal karyotypes.

U2 - 10.1038/nmeth.1200

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Improved genetic manipulation of human embryonic stem cells.

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