In Situ Hybridization to Identify Gut Stem Cells

TY - JOUR

T1 - In Situ Hybridization to Identify Gut Stem Cells

AU - Gregorieff, Alex

AU - Clevers, Hans

N1 - Copyright © 2015 John Wiley & Sons, Inc.

PY - 2015

Y1 - 2015

N2 - In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution. © 2015 by John Wiley & Sons, Inc.

AB - In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution. © 2015 by John Wiley & Sons, Inc.

U2 - 10.1002/9780470151808.sc02f01s34

DO - 10.1002/9780470151808.sc02f01s34

M3 - Article

C2 - 26237569

SN - 1941-7322

VL - 34

SP - 2F.1.1-2F.1.11

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

ER -