In situ hybridization to identify gut stem cells

TY - JOUR

T1 - In situ hybridization to identify gut stem cells

AU - Gregorieff, A.

AU - Clevers, H.

N1 - Reporting year: 2010

PY - 2010

Y1 - 2010

N2 - In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.

AB - In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.

U2 - 10.1002/9780470151808.sc02f01s12

DO - 10.1002/9780470151808.sc02f01s12

M3 - Article

SN - 1941-7322

VL - Chapter 2

SP - 1

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

ER -