In Vitro Activities of Five Antifungal Drugs Against Opportunistic Agents of Aspergillus Nigri Complex

TY - JOUR

T1 - In Vitro Activities of Five Antifungal Drugs Against Opportunistic Agents of Aspergillus Nigri Complex

AU - Badali, H.

AU - Fakhim, H.

AU - Zarei, F.

AU - Nabili, M.

AU - Vaezi, A.

AU - Poorzad, N.

AU - Dolatabadi, S.

AU - Mirhendi, H.

N1 - ISI Document Delivery No.: DG7UU Times Cited: 1 Cited Reference Count: 22 Badali, Hamid Fakhim, Hamed Zarei, Fereshteh Nabili, Mojtaba Vaezi, Afsane Poorzad, Nafiseh Dolatabadi, Somayeh Mirhendi, Hossein School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran [1106] This study was financially supported by a grant from the School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran (no. 1106) which we gratefully acknowledge. We are grateful to Iman Haghani for excellent technical assistance and help with antifungal susceptibility testing. Springer Dordrecht

PY - 2016

Y1 - 2016

N2 - Black aspergilli, particularly Aspergillus niger and A. tubingensis, are the most common etiological agents of otomycosis followed by onychomycosis, pulmonary aspergillosis and aspergilloma. However, so far there is no systematic study on their antifungal susceptibility profiles. A collection of 124 clinical and environmental species of black aspergilli consisted of A. niger, A. tubingensis, A. uvarum. A. acidus and A. sydowii were verified by DNA sequencing of the partial beta-tubulin gene. MICs of amphotericin B, itraconazole, voriconazole, posaconazole, and MECs of caspofungin were performed based on CLSI M38-A2. Posaconazole and caspofungin had the lowest MIC range (0.016-0.125 A mu g/ml and 0.008-0.031 A mu g/ml, respectively), followed by amphotericin B (0.25-4 A mu g/ml), voriconazole (0.125-16 A mu g/ml) and itraconazole (0.25 to > 16) in an increasing order. Some strains of A. niger showed high MIC value for itraconazole and voriconazole (> 16 A mu g/ml), in contrast only environmental isolates of A. tubingensis had high itraconazole MICs (> 16 A mu g/ml). These results confirm that posaconazole and caspofungin are potential drugs for treatment of aspergillosis due to opportunistic agents of Aspergillus Nigri complex. However, in vivo efficacy remains to be determined.

AB - Black aspergilli, particularly Aspergillus niger and A. tubingensis, are the most common etiological agents of otomycosis followed by onychomycosis, pulmonary aspergillosis and aspergilloma. However, so far there is no systematic study on their antifungal susceptibility profiles. A collection of 124 clinical and environmental species of black aspergilli consisted of A. niger, A. tubingensis, A. uvarum. A. acidus and A. sydowii were verified by DNA sequencing of the partial beta-tubulin gene. MICs of amphotericin B, itraconazole, voriconazole, posaconazole, and MECs of caspofungin were performed based on CLSI M38-A2. Posaconazole and caspofungin had the lowest MIC range (0.016-0.125 A mu g/ml and 0.008-0.031 A mu g/ml, respectively), followed by amphotericin B (0.25-4 A mu g/ml), voriconazole (0.125-16 A mu g/ml) and itraconazole (0.25 to > 16) in an increasing order. Some strains of A. niger showed high MIC value for itraconazole and voriconazole (> 16 A mu g/ml), in contrast only environmental isolates of A. tubingensis had high itraconazole MICs (> 16 A mu g/ml). These results confirm that posaconazole and caspofungin are potential drugs for treatment of aspergillosis due to opportunistic agents of Aspergillus Nigri complex. However, in vivo efficacy remains to be determined.

KW - Aspergillus Nigri complex In vitro susceptibility Partial beta-tubulin (BTU) azole resistance black aspergilli section nigri fumigatus susceptibilities onychomycosis otomycosis

U2 - 10.1007/s11046-015-9968-0

DO - 10.1007/s11046-015-9968-0

M3 - Article

VL - 181

SP - 235

EP - 240

JO - Mycopathologia

JF - Mycopathologia

SN - 0301-486X

IS - 3-4

ER -