In Vitro Antifungal Susceptibilities and Amplified Fragment Length Polymorphism Genotyping of a Worldwide Collection of 350 Clinical, Veterinary, and Environmental Cryptococcus gattii Isolates

TY - JOUR

T1 - In Vitro Antifungal Susceptibilities and Amplified Fragment Length Polymorphism Genotyping of a Worldwide Collection of 350 Clinical, Veterinary, and Environmental Cryptococcus gattii Isolates

AU - Hagen, F.

AU - Illnait-Zaragozi, M.T.

AU - Bartlett, K.H.

AU - Swinne, D.

AU - Geertsen, E.

AU - Klaassen, C.H.W.

AU - Boekhout, T.

AU - Meis, J.F.

N1 - Reporting year: 2010

PY - 2010

Y1 - 2010

N2 - The in vitro susceptibilities of a worldwide collection of 350 Cryptococcus gattii isolates to seven antifungal drugs, including the new triazole isavuconazole, were tested. With amplified fragment length polymorphism (AFLP) fingerprinting, human, veterinary, and environmental C. gattii isolates were subdivided into seven AFLP genotypes, including the interspecies hybrids AFLP8 and AFLP9. The majority of clinical isolates (n = 215) comprised genotypes AFLP4 (n = 76) and AFLP6 (n = 103). The clinical AFLP6 isolates had significantly higher geometric mean MICs for flucytosine and fluconazole than the clinical AFLP4 isolates. Of the seven antifungal compounds examined in this study, isavuconazole had the lowest MIC90 (0.125 mu g/ml) for all C. gattii isolates, followed by a 1 log(2) dilution step increase (MIC90, 0.25 mu g/ml) for itraconazole, voriconazole, and posaconazole. Amphotericin B had an acceptable MIC90 of 0.5 mu g/ml, but fluconazole and flucytosine had relatively high MIC(90)s of 8 mu g/ml.

AB - The in vitro susceptibilities of a worldwide collection of 350 Cryptococcus gattii isolates to seven antifungal drugs, including the new triazole isavuconazole, were tested. With amplified fragment length polymorphism (AFLP) fingerprinting, human, veterinary, and environmental C. gattii isolates were subdivided into seven AFLP genotypes, including the interspecies hybrids AFLP8 and AFLP9. The majority of clinical isolates (n = 215) comprised genotypes AFLP4 (n = 76) and AFLP6 (n = 103). The clinical AFLP6 isolates had significantly higher geometric mean MICs for flucytosine and fluconazole than the clinical AFLP4 isolates. Of the seven antifungal compounds examined in this study, isavuconazole had the lowest MIC90 (0.125 mu g/ml) for all C. gattii isolates, followed by a 1 log(2) dilution step increase (MIC90, 0.25 mu g/ml) for itraconazole, voriconazole, and posaconazole. Amphotericin B had an acceptable MIC90 of 0.5 mu g/ml, but fluconazole and flucytosine had relatively high MIC(90)s of 8 mu g/ml.

U2 - 10.1128/AAC.00746-10

DO - 10.1128/AAC.00746-10

M3 - Article

SN - 0066-4804

VL - 54

SP - 5139

EP - 5145

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 12

ER -