TY - CHAP
T1 - Induction of the Sexual Cycle in Filamentous Ascomycetes
AU - Houbraken, Jos
AU - Dyer, Paul S
A2 - Berg, Marco A. van den
A2 - Maruthachalam, Karunakaran
A2 - Berg, Marco A. van den
A2 - Maruthachalam, Karunakaran
PY - 2015
Y1 - 2015
N2 - Sexual reproduction provides a valuable tool for strain development and gene identification in fungi, but has been limited in its use because many economically important fungi are only known to reproduce asexually. However, in the past decade a number of publications have reported sexual cycles in supposedly ‘asexual’ fungi, indicating that many presumed asexual fungi might have the potential for sexual reproduction. Methods are therefore described to induce a sexual cycle in various filamentous fungi, including the use of mating-type gene diagnostics to identify compatible mating types in heterothallic ascomycete species, the use of different crossing conditions, and importance of inoculation technique. The conditions for successful mating are often species-specific and it is therefore difficult to give a general protocol. Different methods for the isolation and analysis of ascospore offspring are then described. Finally, ways in which the sexual cycle can be exploited for purposes including gene identification and localization, strain improvement, and gene complementation are covered. It is anticipated that the application of such methodologies will generate sexual progeny with new characteristics or previously unobserved properties
AB - Sexual reproduction provides a valuable tool for strain development and gene identification in fungi, but has been limited in its use because many economically important fungi are only known to reproduce asexually. However, in the past decade a number of publications have reported sexual cycles in supposedly ‘asexual’ fungi, indicating that many presumed asexual fungi might have the potential for sexual reproduction. Methods are therefore described to induce a sexual cycle in various filamentous fungi, including the use of mating-type gene diagnostics to identify compatible mating types in heterothallic ascomycete species, the use of different crossing conditions, and importance of inoculation technique. The conditions for successful mating are often species-specific and it is therefore difficult to give a general protocol. Different methods for the isolation and analysis of ascospore offspring are then described. Finally, ways in which the sexual cycle can be exploited for purposes including gene identification and localization, strain improvement, and gene complementation are covered. It is anticipated that the application of such methodologies will generate sexual progeny with new characteristics or previously unobserved properties
U2 - 10.1007/978-3-319-10503-1
DO - 10.1007/978-3-319-10503-1
M3 - Chapter
SN - 978-3-319-10502-4
T3 - Genetic Transformation Systems in Fungi, Volume 2
SP - 23
EP - 46
BT - Genetic Transformation Systems in Fungi, Volume 2
PB - Springer International Publishing AG
ER -