Insect pathogenicity in plant-beneficial pseudomonads: phylogenetic distribution and comparative genomics

Pascale Flury, Nora Aellen, Beat Ruffner, Maria Pechy-Tarr, Shakira Fataar, Zane Metla, Ana Dominguez-Ferreras, Guido Bloemberg, Joachim Frey, Alexander Goesmann, Jos M Raaijmakers, Brion Duffy, Monica Hofte, Jochen Blom, Theo H M Smits, Christoph Keel, Monika Maurhofer

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Bacteria of the genus Pseudomonas occupy diverse environments. The Pseudomonas fluorescens group is particularly well-known for its plant-beneficial properties including pathogen suppression. Recent observations that some strains of this group also cause lethal infections in insect larvae, however, point to a more versatile ecology of these bacteria. We show that 26 P. fluorescens group strains, isolated from three continents and covering three phylogenetically distinct sub-clades, exhibited different activities toward lepidopteran larvae, ranging from lethal to avirulent. All strains of sub-clade 1, which includes Pseudomonas chlororaphis and Pseudomonas protegens, were highly insecticidal regardless of their origin (animals, plants). Comparative genomics revealed that strains in this sub-clade possess specific traits allowing a switch between plant- and insect-associated lifestyles. We identified 90 genes unique to all highly insecticidal strains (sub-clade 1) and 117 genes common to all strains of sub-clade 1 and present in some moderately insecticidal strains of sub-clade 3. Mutational analysis of selected genes revealed the importance of chitinase C and phospholipase C in insect pathogenicity. The study provides insight into the genetic basis and phylogenetic distribution of traits defining insecticidal activity in plant-beneficial pseudomonads. Strains with potent dual activity against plant pathogens and herbivorous insects have great potential for use in integrated pest management for crops.
Originele taal-2Engels
Pagina's (van-tot)2527-2542
TijdschriftISME Journal
Volume10
Vroegere onlinedatum19 feb 2016
DOI's
StatusGepubliceerd - 2016

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