TY - JOUR
T1 - Interaction of wheat-Parastagonospora nodorum isolates
T2 - Exploring host susceptibility and fungal virulence
AU - Choupannejad, Roya
AU - Sharifnabi, Bahram
AU - Collemare, Jérôme
AU - Massah, Amir
AU - Gholami, Javad
AU - Mehrabi, Rahim
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2024/3
Y1 - 2024/3
N2 - The fungus Parastagonospora nodorum causes substantial economic losses in wheat worldwide. P. nodorum secretes multiple proteinaceous necrotrophic effectors (NEs) that induce compatible interactions with hosts possessing corresponding dominant susceptibility (S) genes. This study focused on unraveling the virulence pattern and presence of NEs secreted by 33 isolates of P. nodorum in the context of its interaction with 40 wheat genotypes. Wheat genotypes were classified into six groups based on their variable responses to P. nodorum isolates. The S gene Tsn1 was present in 70 % of the highly susceptible cultivars and completely absent in all resistant cultivars. Cultivars possessing all three S genes, Tsn1, Snn1, and Snn3, were susceptible to all P. nodorum isolates. The S gene Snn3 was detected in all Iranian wheat cultivars, whereas its proportion in the world's cultivars was 62.5 %. SnTox1-Snn1 was the most abundant NE-S gene (100 %) in the Iranian P. nodorum and Iranian cultivars. Furthermore, SnToxA was produced in Pichia pastoris, and infiltration of SnToxA resulted in necrosis response exclusively in wheat genotypes harboring the S gene Tsn1. Overall, these findings extend our substantial knowledge of P. nodorum-wheat interactions and their underlying molecular basis in Iran, a country in the Fertile Crescent where both wheat and its pathogens coevolved.
AB - The fungus Parastagonospora nodorum causes substantial economic losses in wheat worldwide. P. nodorum secretes multiple proteinaceous necrotrophic effectors (NEs) that induce compatible interactions with hosts possessing corresponding dominant susceptibility (S) genes. This study focused on unraveling the virulence pattern and presence of NEs secreted by 33 isolates of P. nodorum in the context of its interaction with 40 wheat genotypes. Wheat genotypes were classified into six groups based on their variable responses to P. nodorum isolates. The S gene Tsn1 was present in 70 % of the highly susceptible cultivars and completely absent in all resistant cultivars. Cultivars possessing all three S genes, Tsn1, Snn1, and Snn3, were susceptible to all P. nodorum isolates. The S gene Snn3 was detected in all Iranian wheat cultivars, whereas its proportion in the world's cultivars was 62.5 %. SnTox1-Snn1 was the most abundant NE-S gene (100 %) in the Iranian P. nodorum and Iranian cultivars. Furthermore, SnToxA was produced in Pichia pastoris, and infiltration of SnToxA resulted in necrosis response exclusively in wheat genotypes harboring the S gene Tsn1. Overall, these findings extend our substantial knowledge of P. nodorum-wheat interactions and their underlying molecular basis in Iran, a country in the Fertile Crescent where both wheat and its pathogens coevolved.
KW - NE infiltration
KW - Necrotrophic effector
KW - Pathogenesis pattern
KW - Susceptibility gene
KW - Wheat leaf and glume blotch
UR - http://www.scopus.com/inward/record.url?scp=85183302853&partnerID=8YFLogxK
U2 - 10.1016/j.pmpp.2024.102235
DO - 10.1016/j.pmpp.2024.102235
M3 - Article
AN - SCOPUS:85183302853
SN - 0885-5765
VL - 130
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
M1 - 102235
ER -