Intra- and interspecies virus transfer in Aspergilli via protoplast fusion

TY - JOUR

T1 - Intra- and interspecies virus transfer in Aspergilli via protoplast fusion

AU - van Diepeningen, A D

AU - Debets, A J

AU - Hoekstra, R F

N1 - Copyright 1998 Academic Press.

PY - 1998/12

Y1 - 1998/12

N2 - Intra- and interspecies transfer of dsRNA viruses between black Aspergilli and Aspergillus nidulans strains has been investigated using protoplast fusion. We found interspecies transfer of virus in all combinations of black Aspergillus and A. nidulans strains and vice versa. Using the same conditions, intraspecies virus transfer among heterokaryon incompatible strains was also tested. Whereas such transfer was always found among A. nidulans strains, transfer among black Aspergilli was frequently unsuccessful. The lack of virus transfer between black Aspergillus isolates was further investigated by using a mitochondrial oligomycin resistance marker as a positive control for cytoplasmic exchange. These experiments showed independent transfer of the oligomycin resistance and dsRNA viruses during protoplast fusion of heterokaryon incompatible black Aspergilli. The inefficient transfer of dsRNA viruses between black Aspergilli is not caused by absolute resistance to viruses but may be related to heterokaryon incompatibility reactions that operate intraspecifically. Consequences for the dynamics of mycoviruses in populations of black Aspergilli are discussed.

AB - Intra- and interspecies transfer of dsRNA viruses between black Aspergilli and Aspergillus nidulans strains has been investigated using protoplast fusion. We found interspecies transfer of virus in all combinations of black Aspergillus and A. nidulans strains and vice versa. Using the same conditions, intraspecies virus transfer among heterokaryon incompatible strains was also tested. Whereas such transfer was always found among A. nidulans strains, transfer among black Aspergilli was frequently unsuccessful. The lack of virus transfer between black Aspergillus isolates was further investigated by using a mitochondrial oligomycin resistance marker as a positive control for cytoplasmic exchange. These experiments showed independent transfer of the oligomycin resistance and dsRNA viruses during protoplast fusion of heterokaryon incompatible black Aspergilli. The inefficient transfer of dsRNA viruses between black Aspergilli is not caused by absolute resistance to viruses but may be related to heterokaryon incompatibility reactions that operate intraspecifically. Consequences for the dynamics of mycoviruses in populations of black Aspergilli are discussed.

KW - Anti-Bacterial Agents

KW - Aspergillus

KW - Aspergillus nidulans

KW - Cell Nucleus

KW - Drug Resistance, Microbial

KW - Membrane Fusion

KW - Oligomycins

KW - Protoplasts

KW - RNA Viruses

KW - RNA, Double-Stranded

KW - Recombination, Genetic

KW - Species Specificity

U2 - 10.1006/fgbi.1998.1096

DO - 10.1006/fgbi.1998.1096

M3 - Article

C2 - 9917371

SN - 1087-1845

VL - 25

SP - 171

EP - 180

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

IS - 3

ER -