One hundred and thirty-three clinical Trichosporon isolates were collected in the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program (2009 to 2016). Accurate identification was performed by sequencing of the intergenic spacer (IGS) 1 region. Among these isolates T. asahii (108, 81.2%) was the leading species, followed by T. dermatis (7, 5.3%), T. asteroides (5, 3.8%), T. inkin (5, 3.8%), T. dohaense (3, 2.3%), and one (0.7%) each of T. faecale, T. jirovecii, T. mucoides, T. coremiiforme and T. montevideense Both the VITEK MS (bioMérieux, Marcy l'Etoile, France) and Bruker Biotyper MS (Bruker Daltonics GmbH, Germany) platforms gave a high level (>97.5%) of correct identification when the species were present in the database. Geometric mean (GM) of amphotericin B MICs for T. asahii was 2-fold higher than for non-asahii Trichosporon High fluconazole MICs (≥8 μg/ml) were observed in 25% (27/108) of T. asahii and 16% (4/25) of non-asahii Trichosporon isolates. Itraconazole MICs were ≤0.5 μg/ml for 89.5% of the isolates. Voriconazole was the most potent antifungal agent in vitro, with GM of 0.09 μg/ml. Genotyping of the isolates using the IGS1 sequences alignment revealed that genotype1 was most common (41.7%), followed by genotype 4 (31.5%), 3 (23.1%), 5 (0.9%), 6 (0.9%) and 7 (1.8%). Our data of species distribution, genotypes and antifungal susceptibilities may contribute to a better understanding of the epidemiology of invasive Trichosporon infections throughout China.