Isolation and characterization of microsatellite loci in Cylindrocladium pauciramosum

TY - JOUR

T1 - Isolation and characterization of microsatellite loci in Cylindrocladium pauciramosum

AU - Wright, L. P.

AU - Wingfield, B.D.

AU - Wingfield, M.J.

AU - Crous, P.W.

N1 - doi:10.1111/j.1471-8286.2006.01603.x

PY - 2007

Y1 - 2007

N2 - Ten polymorphic microsatellite markers were developed for Cylindrocladium pauciramosum, a plant pathogen with a wide host range, which poses a serious problem in South African Eucalyptus nurseries. Polymorphism was evaluated on 43 isolates collected from Colombia and South Africa. Each locus had between three and six alleles. Testing for random mating showed multilocus equilibrium for a population of 40 isolates from a South African forestry nursery. Cross-species transferability tested for 19 other Cylindrocladium species found amplification only in C. spathulatum, which is phylogenetically closely related to C. pauciramosum.

AB - Ten polymorphic microsatellite markers were developed for Cylindrocladium pauciramosum, a plant pathogen with a wide host range, which poses a serious problem in South African Eucalyptus nurseries. Polymorphism was evaluated on 43 isolates collected from Colombia and South Africa. Each locus had between three and six alleles. Testing for random mating showed multilocus equilibrium for a population of 40 isolates from a South African forestry nursery. Cross-species transferability tested for 19 other Cylindrocladium species found amplification only in C. spathulatum, which is phylogenetically closely related to C. pauciramosum.

M3 - Article

SN - 1471-8286

VL - 7

SP - 343

EP - 345

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

ER -