Isolation and in vitro expansion of human colonic stem cells

P. Jung; T. Sato; A. Merlos-Suarez; F.M. Barriga; M. Iglesias; D. Rossell; H. Auer; M. Gallardo; M.A. Blasco; E. Sancho; H. Clevers; E. Batlle

doi:10.1038/nm.2470

Isolation and in vitro expansion of human colonic stem cells

P. Jung, T. Sato, A. Merlos-Suarez, F.M. Barriga, M. Iglesias, D. Rossell, H. Auer, M. Gallardo, M.A. Blasco, E. Sancho, H. Clevers, E. Batlle

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

566 Citaten (Scopus)

Samenvatting

Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population. [KEYWORDS: Cell Differentiation/physiology, Cell Proliferation, Colon/ cytology, Flow Cytometry, Humans, Immunohistochemistry, Intestinal Mucosa/ cytology, Microscopy, Confocal, Multipotent Stem Cells/cytology/ physiology, Real-Time Polymerase Chain Reaction, Receptor, EphB2/ metabolism, Telomere/metabolism]

Originele taal-2	Engels
Pagina's (van-tot)	1225-1227
Tijdschrift	Nature Medicine
Volume	17
Nummer van het tijdschrift	10
DOI's	https://doi.org/10.1038/nm.2470
Status	Gepubliceerd - 2011

Toegang tot document

10.1038/nm.2470

Citeer dit

@article{def4c21640304dd9a959e2501458d8aa,

title = "Isolation and in vitro expansion of human colonic stem cells",

abstract = "Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population. [KEYWORDS: Cell Differentiation/physiology, Cell Proliferation, Colon/ cytology, Flow Cytometry, Humans, Immunohistochemistry, Intestinal Mucosa/ cytology, Microscopy, Confocal, Multipotent Stem Cells/cytology/ physiology, Real-Time Polymerase Chain Reaction, Receptor, EphB2/ metabolism, Telomere/metabolism]",

author = "P. Jung and T. Sato and A. Merlos-Suarez and F.M. Barriga and M. Iglesias and D. Rossell and H. Auer and M. Gallardo and M.A. Blasco and E. Sancho and H. Clevers and E. Batlle",

note = "Reporting year: 2011",

year = "2011",

doi = "10.1038/nm.2470",

language = "English",

volume = "17",

pages = "1225--1227",

journal = "Nature Medicine",

issn = "1078-8956",

publisher = "Nature Publishing Group",

number = "10",

}

TY - JOUR

T1 - Isolation and in vitro expansion of human colonic stem cells

AU - Jung, P.

AU - Sato, T.

AU - Merlos-Suarez, A.

AU - Barriga, F.M.

AU - Iglesias, M.

AU - Rossell, D.

AU - Auer, H.

AU - Gallardo, M.

AU - Blasco, M.A.

AU - Sancho, E.

AU - Clevers, H.

AU - Batlle, E.

N1 - Reporting year: 2011

PY - 2011

Y1 - 2011

N2 - Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population. [KEYWORDS: Cell Differentiation/physiology, Cell Proliferation, Colon/ cytology, Flow Cytometry, Humans, Immunohistochemistry, Intestinal Mucosa/ cytology, Microscopy, Confocal, Multipotent Stem Cells/cytology/ physiology, Real-Time Polymerase Chain Reaction, Receptor, EphB2/ metabolism, Telomere/metabolism]

AB - Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population. [KEYWORDS: Cell Differentiation/physiology, Cell Proliferation, Colon/ cytology, Flow Cytometry, Humans, Immunohistochemistry, Intestinal Mucosa/ cytology, Microscopy, Confocal, Multipotent Stem Cells/cytology/ physiology, Real-Time Polymerase Chain Reaction, Receptor, EphB2/ metabolism, Telomere/metabolism]

U2 - 10.1038/nm.2470

DO - 10.1038/nm.2470

M3 - Article

SN - 1078-8956

VL - 17

SP - 1225

EP - 1227

JO - Nature Medicine

JF - Nature Medicine

IS - 10

ER -

Isolation and in vitro expansion of human colonic stem cells

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit