Isolation and in vitro expansion of human colonic stem cells

P. Jung, T. Sato, A. Merlos-Suarez, F.M. Barriga, M. Iglesias, D. Rossell, H. Auer, M. Gallardo, M.A. Blasco, E. Sancho, H. Clevers, E. Batlle

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population. [KEYWORDS: Cell Differentiation/physiology, Cell Proliferation, Colon/ cytology, Flow Cytometry, Humans, Immunohistochemistry, Intestinal Mucosa/ cytology, Microscopy, Confocal, Multipotent Stem Cells/cytology/ physiology, Real-Time Polymerase Chain Reaction, Receptor, EphB2/ metabolism, Telomere/metabolism]
Originele taal-2Engels
Pagina's (van-tot)1225-1227
TijdschriftNature Medicine
Volume17
Nummer van het tijdschrift10
DOI's
StatusGepubliceerd - 2011

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