Isolation of deletion alleles by G4 DNA-induced mutagenesis.

TY - JOUR

T1 - Isolation of deletion alleles by G4 DNA-induced mutagenesis.

AU - Pontier, D.B.

AU - Kruisselbrink, E.

AU - Guryev, V.

AU - Tijsterman, M.

N1 - Reporting year: 2009

PY - 2009

Y1 - 2009

N2 - Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.

AB - Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.

U2 - 10.1038/NMETH.1362

DO - 10.1038/NMETH.1362

M3 - Article

SN - 1548-7091

VL - 6

SP - 655

EP - 657

JO - Nature Methods

JF - Nature Methods

IS - 9

ER -