Knock-Out Serum Replacement and Melatonin Effects on Germ Cell Differentiation in Murine Testicular Explant Cultures

Ahmed Reda, Halima Albalushi, Sheyla Cisneros Montalvo, Mirja Nurmio, Zeliha Sahin, Mi Hou, Niels Geijsen, Jorma Toppari, Olle Söder, Jan-Bernd Stukenborg

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Finding robust culture conditions for in vitro maturation (IVM) of male germ cells is still a challenge. Recently, a testis organ culture method, using Knockout Serum Replacement (KSR), was suggested as a promising approach. However, the efficiency of that model is still not optimal. Hence, we have tried to establish the culture conditions in two laboratories, and to improve the reliability of the culture system to generate mature germ cells. Male mice at three days of age were sacrificed. Testes were cut into small pieces which were cultured atop agarose stands, using Minimum Essential Medium alpha supplemented with different supplements; melatonin, Glutamax, and different concentrations of KSR. The results showed that the duration of culture beyond 18 days had an impact on the number of differentiated germ cells. Supplementation with melatonin and Glutamax revealed a positive influence on the efficiency of male germ cell differentiation in vitro. Furthermore, the results confirmed that KSR had a positive effect on germ cell maturation and testosterone production, with a concentration of at least 10%. In conclusion, this study emphasizes the beneficial role of at least 10% KSR in the IVM of germ cells.

Originele taal-2Engels
Pagina's (van-tot)1783-1794
Aantal pagina's12
TijdschriftAnnals of Biomedical Engineering
Volume45
Nummer van het tijdschrift7
DOI's
StatusGepubliceerd - jul 2017

Vingerafdruk

Duik in de onderzoeksthema's van 'Knock-Out Serum Replacement and Melatonin Effects on Germ Cell Differentiation in Murine Testicular Explant Cultures'. Samen vormen ze een unieke vingerafdruk.

Citeer dit