Kondoa qatarensis f.a., sp. nov., a novel yeast species isolated from marine water in Qatar

TY - JOUR

T1 - Kondoa qatarensis f.a., sp. nov., a novel yeast species isolated from marine water in Qatar

AU - Fotedar, Rashmi

AU - Kolecka, Anna

AU - Boekhout, Teun

AU - Fell, Jack W

AU - Zeyara, Aisha

AU - Al Malki, Ameena

AU - Al Marri, Masoud

PY - 2019/1/9

Y1 - 2019/1/9

N2 - During a study of yeast diversity in marine waters of the Arabian Gulf surrounding Qatar, 30 strains were isolated that represent a novel species of Kondoa. The isolates were obtained from five locations along the Qatari coast. Species identifications were based on sequence analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the large subunit (LSU) rRNA gene. Five strains were selected for further study. The novel species was closely related to Kondoa aeria (CBS 8352T) and Kondoa malvinella (CBS 6082T) but differed from the two species by 5.6 % sequence divergence (16 substitutions and 14 gaps) in the ITS region. On the basis of D1/D2 domains of the LSU rRNA gene, the novel species differed from K. aeria by only >0.35 % sequence divergence (2 substitutions and 0 gaps) and 1.5 % sequence divergence (9 substitutions and 1 gap) to that of K. malvinella. Although the novel species showed a close similarity in the D1/D2 domains to K. aeria (CBS 8352T), based on the significant differences (16 substitutions) in ITS regions and on the basis of physiological and biochemical tests, viz ability to grow at 35 °C, and inability to assimilate, sucrose, raffinose, d,l-lactate, succinate, citrate and nitrite, these strains are considered as novel species of Kondoa. The species name of Kondoa qatarensis f.a., sp. nov. is proposed with specimen 2Y109 as the holotype.

AB - During a study of yeast diversity in marine waters of the Arabian Gulf surrounding Qatar, 30 strains were isolated that represent a novel species of Kondoa. The isolates were obtained from five locations along the Qatari coast. Species identifications were based on sequence analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the large subunit (LSU) rRNA gene. Five strains were selected for further study. The novel species was closely related to Kondoa aeria (CBS 8352T) and Kondoa malvinella (CBS 6082T) but differed from the two species by 5.6 % sequence divergence (16 substitutions and 14 gaps) in the ITS region. On the basis of D1/D2 domains of the LSU rRNA gene, the novel species differed from K. aeria by only >0.35 % sequence divergence (2 substitutions and 0 gaps) and 1.5 % sequence divergence (9 substitutions and 1 gap) to that of K. malvinella. Although the novel species showed a close similarity in the D1/D2 domains to K. aeria (CBS 8352T), based on the significant differences (16 substitutions) in ITS regions and on the basis of physiological and biochemical tests, viz ability to grow at 35 °C, and inability to assimilate, sucrose, raffinose, d,l-lactate, succinate, citrate and nitrite, these strains are considered as novel species of Kondoa. The species name of Kondoa qatarensis f.a., sp. nov. is proposed with specimen 2Y109 as the holotype.

U2 - 10.1099/ijsem.0.003182

DO - 10.1099/ijsem.0.003182

M3 - Article

C2 - 30625110

SN - 1466-5026

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

ER -