Laser photoacoustic trace gas detection, an extremely sensitive technique applied in biological research

S. te Lintel Hekkert, M.J. Staal, R.H.M. Nabben, H. Zuckermann, S. Persijn, L.J. Stal, L.A.C.J. Voesenek, F.J.M. Harren, J. Reuss, D.H. Parker

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    The use of infrared laser based photo-acoustic trace gas detection equipment in biological research is discussed on the basis bf two examples. A CO2 laser based photo-acoustic trace gas detection system is employed to follow the time-dependent pattern of the nitrogen fixation process by the cyanobacteria Nodularia Spumigena on a one-minute time scale. Due to the high sensitivity of the detection system for ethylene (detection limit 6 part per trillion; 6.10(12)), the fixation process can be followed on-line in a flow-through system. Following a 50 h dark incubation period, the bacteria show nitrogen fixation only after a certain illumination period, indicating lack of carbohydrates needed to start the nitrogen fixation. [KEYWORDS: CO-LASER; PLANTS; ACETALDEHYDE; SPECTROSCOPY; ADAPTATIONS; CO2-LASER; RESPONSES; ETHYLENE; ECOLOGY; ANOXIA]
    Originele taal-2Engels
    Pagina's (van-tot)157-175
    TijdschriftInstrumentation Science and Technology
    Volume26
    Nummer van het tijdschrift2-3
    DOI's
    StatusGepubliceerd - 1998

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