Lettuce and rhizosphere microbiome responses to growth promoting Pseudomonas species under field conditions

TY - JOUR

T1 - Lettuce and rhizosphere microbiome responses to growth promoting Pseudomonas species under field conditions

AU - Cipriano, M.A.P.

AU - Lupatini, M.

AU - Santos, L.

AU - Silva, M. da

AU - Roesch, L.F.W.

AU - Destefano, S.

AU - Freitas, S.

AU - Kuramae, E.E.

N1 - 6166, ME; Data archiving: data archived at ENA (accession number PRJEB12505)

PY - 2016/12

Y1 - 2016/12

N2 - Plant growth promoting rhizobacteria (PGPR) are well described and recommended for several crops worldwide. However, one of the most common problems in PGPR research is the difficulty in obtaining reproducible results. Furthermore, few studies have evaluated plant growth promotion and soil microbial community composition due to bacterial inoculation under field conditions. Here we evaluated the effect of 54 Pseudomonas strains on lettuce (Lactuca sativa) growth. The 12 most promising strains were phylogenetically and physiologically characterized for plant growth-promoting traits including phosphate solubilization, hormone production and antagonism to pathogen compounds and their effect on plant growth under farm field conditions. Additionally, the impact of beneficial strains on the rhizospheric bacterial community was evaluated for inoculated plants. The strains IAC-RBcr4 and IAC-RBru1, with different plant growth promoting traits, improved lettuce plant biomass yields up to 30%. These two strains also impacted rhizosphere bacterial groups including Isosphaera and Pirellula (phylum Planctomycetes) and Acidothermus, Pseudolabrys and Singusphaera (phylum Actinobacteria). This is the first study to demonstrate consistent results for the effects of Pseudomonas strains on lettuce growth promotion for seedlings and plants grown under tropical field conditions.

AB - Plant growth promoting rhizobacteria (PGPR) are well described and recommended for several crops worldwide. However, one of the most common problems in PGPR research is the difficulty in obtaining reproducible results. Furthermore, few studies have evaluated plant growth promotion and soil microbial community composition due to bacterial inoculation under field conditions. Here we evaluated the effect of 54 Pseudomonas strains on lettuce (Lactuca sativa) growth. The 12 most promising strains were phylogenetically and physiologically characterized for plant growth-promoting traits including phosphate solubilization, hormone production and antagonism to pathogen compounds and their effect on plant growth under farm field conditions. Additionally, the impact of beneficial strains on the rhizospheric bacterial community was evaluated for inoculated plants. The strains IAC-RBcr4 and IAC-RBru1, with different plant growth promoting traits, improved lettuce plant biomass yields up to 30%. These two strains also impacted rhizosphere bacterial groups including Isosphaera and Pirellula (phylum Planctomycetes) and Acidothermus, Pseudolabrys and Singusphaera (phylum Actinobacteria). This is the first study to demonstrate consistent results for the effects of Pseudomonas strains on lettuce growth promotion for seedlings and plants grown under tropical field conditions.

KW - international

UR - https://www.ebi.ac.uk/ena/data/view/PRJEB12505

U2 - 10.1093/femsec/fiw197

DO - 10.1093/femsec/fiw197

M3 - Article

SN - 0168-6496

VL - 92

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 12

M1 - fiw197

ER -