Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro

N. Barker; M. Huch; P. Kujala; M.L. van de Wetering; H.J.G. Snippert; J.H. van Es; T. Sato; D.E. Stange; H.L. Begthel; M.M.W. van den Born; E.M. Danenberg; S. van den Brink; J. Korving; A. Abo; P. Peters; N. Wright; R. Poulsom; H. Clevers

doi:10.1016/j.stem.2009.11.013

Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro

N. Barker, M. Huch, P. Kujala, M.L. van de Wetering, H.J.G. Snippert, J.H. van Es, T. Sato, D.E. Stange, H.L. Begthel, M.M.W. van den Born, E.M. Danenberg, S. van den Brink, J. Korving, A. Abo, P. Peters, N. Wright, R. Poulsom, H. Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

1256 Citaten (Scopus)

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The study of gastric epithelial homeostasis and cancer has been hampered by the lack of stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in the small intestine, colon, and hair follicle. Here, we investigated Lgr5 expression in the stomach and assessed the stem cell potential of the Lgr5(+ve) cells by using in vivo lineage tracing. In neonatal stomach, Lgr5 was expressed at the base of prospective corpus and pyloric glands, whereas expression in the adult was predominantly restricted to the base of mature pyloric glands. Lineage tracing revealed these Lgr5(+ve) cells to be self-renewing, multipotent stem cells responsible for the long-term renewal of the gastric epithelium. With an in vitro culture system, single Lgr5(+ve) cells efficiently generated long-lived organoids resembling mature pyloric epithelium. The Lgr5 stem cell marker and culture method described here will be invaluable tools for accelerating research into gastric epithelial renewal, inflammation/infection, and cancer.

Originele taal-2	Engels
Pagina's (van-tot)	25-36
Tijdschrift	Cell Stem Cell
Volume	6
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1016/j.stem.2009.11.013
Status	Gepubliceerd - 2010

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10.1016/j.stem.2009.11.013

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Barker, N., Huch, M., Kujala, P., van de Wetering, M. L., Snippert, H. J. G., van Es, J. H., Sato, T., Stange, D. E., Begthel, H. L., van den Born, M. M. W., Danenberg, E. M., van den Brink, S., Korving, J., Abo, A., Peters, P., Wright, N., Poulsom, R., & Clevers, H. (2010). Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell, 6(1), 25-36. https://doi.org/10.1016/j.stem.2009.11.013

@article{4b7b556c3e934f768606d8a4a20f65d9,

title = "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro",

abstract = "The study of gastric epithelial homeostasis and cancer has been hampered by the lack of stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in the small intestine, colon, and hair follicle. Here, we investigated Lgr5 expression in the stomach and assessed the stem cell potential of the Lgr5(+ve) cells by using in vivo lineage tracing. In neonatal stomach, Lgr5 was expressed at the base of prospective corpus and pyloric glands, whereas expression in the adult was predominantly restricted to the base of mature pyloric glands. Lineage tracing revealed these Lgr5(+ve) cells to be self-renewing, multipotent stem cells responsible for the long-term renewal of the gastric epithelium. With an in vitro culture system, single Lgr5(+ve) cells efficiently generated long-lived organoids resembling mature pyloric epithelium. The Lgr5 stem cell marker and culture method described here will be invaluable tools for accelerating research into gastric epithelial renewal, inflammation/infection, and cancer.",

author = "N. Barker and M. Huch and P. Kujala and {van de Wetering}, M.L. and H.J.G. Snippert and {van Es}, J.H. and T. Sato and D.E. Stange and H.L. Begthel and {van den Born}, M.M.W. and E.M. Danenberg and {van den Brink}, S. and J. Korving and A. Abo and P. Peters and N. Wright and R. Poulsom and H. Clevers",

note = "Reporting year: 2010 Metis note: S1934-5909(09)00618-3 [pii]",

year = "2010",

doi = "10.1016/j.stem.2009.11.013",

language = "English",

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pages = "25--36",

journal = "Cell Stem Cell",

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Barker, N, Huch, M, Kujala, P, van de Wetering, ML, Snippert, HJG, van Es, JH, Sato, T, Stange, DE, Begthel, HL, van den Born, MMW, Danenberg, EM, van den Brink, S, Korving, J, Abo, A, Peters, P, Wright, N, Poulsom, R & Clevers, H 2010, 'Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro', Cell Stem Cell, vol. 6, nr. 1, blz. 25-36. https://doi.org/10.1016/j.stem.2009.11.013

TY - JOUR

T1 - Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro

AU - Barker, N.

AU - Huch, M.

AU - Kujala, P.

AU - van de Wetering, M.L.

AU - Snippert, H.J.G.

AU - van Es, J.H.

AU - Sato, T.

AU - Stange, D.E.

AU - Begthel, H.L.

AU - van den Born, M.M.W.

AU - Danenberg, E.M.

AU - van den Brink, S.

AU - Korving, J.

AU - Abo, A.

AU - Peters, P.

AU - Wright, N.

AU - Poulsom, R.

AU - Clevers, H.

N1 - Reporting year: 2010 Metis note: S1934-5909(09)00618-3 [pii]

PY - 2010

Y1 - 2010

N2 - The study of gastric epithelial homeostasis and cancer has been hampered by the lack of stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in the small intestine, colon, and hair follicle. Here, we investigated Lgr5 expression in the stomach and assessed the stem cell potential of the Lgr5(+ve) cells by using in vivo lineage tracing. In neonatal stomach, Lgr5 was expressed at the base of prospective corpus and pyloric glands, whereas expression in the adult was predominantly restricted to the base of mature pyloric glands. Lineage tracing revealed these Lgr5(+ve) cells to be self-renewing, multipotent stem cells responsible for the long-term renewal of the gastric epithelium. With an in vitro culture system, single Lgr5(+ve) cells efficiently generated long-lived organoids resembling mature pyloric epithelium. The Lgr5 stem cell marker and culture method described here will be invaluable tools for accelerating research into gastric epithelial renewal, inflammation/infection, and cancer.

AB - The study of gastric epithelial homeostasis and cancer has been hampered by the lack of stem cell markers and in vitro culture methods. The Wnt target gene Lgr5 marks stem cells in the small intestine, colon, and hair follicle. Here, we investigated Lgr5 expression in the stomach and assessed the stem cell potential of the Lgr5(+ve) cells by using in vivo lineage tracing. In neonatal stomach, Lgr5 was expressed at the base of prospective corpus and pyloric glands, whereas expression in the adult was predominantly restricted to the base of mature pyloric glands. Lineage tracing revealed these Lgr5(+ve) cells to be self-renewing, multipotent stem cells responsible for the long-term renewal of the gastric epithelium. With an in vitro culture system, single Lgr5(+ve) cells efficiently generated long-lived organoids resembling mature pyloric epithelium. The Lgr5 stem cell marker and culture method described here will be invaluable tools for accelerating research into gastric epithelial renewal, inflammation/infection, and cancer.

U2 - 10.1016/j.stem.2009.11.013

DO - 10.1016/j.stem.2009.11.013

M3 - Article

SN - 1934-5909

VL - 6

SP - 25

EP - 36

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 1

ER -

Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro

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