Live imaging of cell division in 3D stem-cell organoid cultures

TY - JOUR

T1 - Live imaging of cell division in 3D stem-cell organoid cultures

AU - Bolhaqueiro, Ana C F

AU - van Jaarsveld, Richard H

AU - Ponsioen, Bas

AU - Overmeer, René M

AU - Snippert, Hugo J

AU - Kops, Geert J P L

N1 - © 2018 Elsevier Inc. All rights reserved.

PY - 2018

Y1 - 2018

N2 - Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.

AB - Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.

KW - Animals

KW - Cell Culture Techniques/methods

KW - Cell Division/physiology

KW - Humans

KW - Microscopy/methods

KW - Organoids/physiology

KW - Stem Cells/physiology

U2 - 10.1016/bs.mcb.2018.03.016

DO - 10.1016/bs.mcb.2018.03.016

M3 - Article

C2 - 29957217

SN - 0091-679X

VL - 145

SP - 91

EP - 106

JO - Methods in Cell Biology

JF - Methods in Cell Biology

ER -