Live imaging of mRNA using RNA-stabilized fluorogenic proteins

TY - JOUR

T1 - Live imaging of mRNA using RNA-stabilized fluorogenic proteins

AU - Wu, Jiahui

AU - Zaccara, Sara

AU - Khuperkar, Deepak

AU - Kim, Hyaeyeong

AU - Tanenbaum, Marvin E

AU - Jaffrey, Samie R

PY - 2019/9

Y1 - 2019/9

N2 - Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.

AB - Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.

KW - Aptamers, Nucleotide/genetics

KW - Bacterial Proteins/genetics

KW - Fluorescence

KW - Fluorescent Dyes/chemistry

KW - HEK293 Cells

KW - Humans

KW - Luminescent Proteins/genetics

KW - Microscopy, Fluorescence/methods

KW - Molecular Imaging/methods

KW - RNA, Messenger/analysis

U2 - 10.1038/s41592-019-0531-7

DO - 10.1038/s41592-019-0531-7

M3 - Article

C2 - 31471614

SN - 1548-7091

VL - 16

SP - 862

EP - 865

JO - Nature Methods

JF - Nature Methods

IS - 9

ER -