Long-term expanding human airway organoids for disease modeling

Norman Sachs; Angelos Papaspyropoulos; Domenique D Zomer-van Ommen; Inha Heo; Lena Böttinger; Dymph Klay; Fleur Weeber; Guizela Huelsz-Prince; Nino Iakobachvili; Gimano D Amatngalim; Joep de Ligt; Arne van Hoeck; Natalie Proost; Marco C Viveen; Anna Lyubimova; Luc Teeven; Sepideh Derakhshan; Jeroen Korving; Harry Begthel; Johanna F Dekkers; Kuldeep Kumawat; Emilio Ramos; Matthijs Fm van Oosterhout; G Johan Offerhaus; Dominique J Wiener; Eduardo P Olimpio; Krijn K Dijkstra; Egbert F Smit; Maarten van der Linden; Sridevi Jaksani; Marieke van de Ven; Jos Jonkers; Anne C Rios; Emile E Voest; Coline Hm van Moorsel; Cornelis K van der Ent; Edwin Cuppen; Alexander van Oudenaarden; Frank E Coenjaerts; Linde Meyaard; Louis J Bont; Peter J Peters; Sander J Tans; Jeroen S van Zon; Sylvia F Boj; Robert G Vries; Jeffrey M Beekman; Hans Clevers

doi:10.15252/embj.2018100300

Long-term expanding human airway organoids for disease modeling

Norman Sachs, Angelos Papaspyropoulos, Domenique D Zomer-van Ommen, Inha Heo, Lena Böttinger, Dymph Klay, Fleur Weeber, Guizela Huelsz-Prince, Nino Iakobachvili, Gimano D Amatngalim, Joep de Ligt, Arne van Hoeck, Natalie Proost, Marco C Viveen, Anna Lyubimova, Luc Teeven, Sepideh Derakhshan, Jeroen Korving, Harry Begthel, Johanna F DekkersKuldeep Kumawat, Emilio Ramos, Matthijs Fm van Oosterhout, G Johan Offerhaus, Dominique J Wiener, Eduardo P Olimpio, Krijn K Dijkstra, Egbert F Smit, Maarten van der Linden, Sridevi Jaksani, Marieke van de Ven, Jos Jonkers, Anne C Rios, Emile E Voest, Coline Hm van Moorsel, Cornelis K van der Ent, Edwin Cuppen, Alexander van Oudenaarden, Frank E Coenjaerts, Linde Meyaard, Louis J Bont, Peter J Peters, Sander J Tans, Jeroen S van Zon, Sylvia F Boj, Robert G Vries, Jeffrey M Beekman, Hans Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

586 Citaten (Scopus)

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Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease.

Originele taal-2	Engels
Tijdschrift	EMBO Journal
Volume	38
Nummer van het tijdschrift	4
DOI's	https://doi.org/10.15252/embj.2018100300
Status	Gepubliceerd - 15 feb. 2019

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Sachs, N., Papaspyropoulos, A., Zomer-van Ommen, D. D., Heo, I., Böttinger, L., Klay, D., Weeber, F., Huelsz-Prince, G., Iakobachvili, N., Amatngalim, G. D., de Ligt, J., van Hoeck, A., Proost, N., Viveen, M. C., Lyubimova, A., Teeven, L., Derakhshan, S., Korving, J., Begthel, H., ... Clevers, H. (2019). Long-term expanding human airway organoids for disease modeling. EMBO Journal, 38(4). https://doi.org/10.15252/embj.2018100300

@article{2390185f75bf48e98da0b2259ba4c5d0,

title = "Long-term expanding human airway organoids for disease modeling",

abstract = "Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease.",

author = "Norman Sachs and Angelos Papaspyropoulos and {Zomer-van Ommen}, {Domenique D} and Inha Heo and Lena B{\"o}ttinger and Dymph Klay and Fleur Weeber and Guizela Huelsz-Prince and Nino Iakobachvili and Amatngalim, {Gimano D} and {de Ligt}, Joep and {van Hoeck}, Arne and Natalie Proost and Viveen, {Marco C} and Anna Lyubimova and Luc Teeven and Sepideh Derakhshan and Jeroen Korving and Harry Begthel and Dekkers, {Johanna F} and Kuldeep Kumawat and Emilio Ramos and {van Oosterhout}, {Matthijs Fm} and Offerhaus, {G Johan} and Wiener, {Dominique J} and Olimpio, {Eduardo P} and Dijkstra, {Krijn K} and Smit, {Egbert F} and {van der Linden}, Maarten and Sridevi Jaksani and {van de Ven}, Marieke and Jos Jonkers and Rios, {Anne C} and Voest, {Emile E} and {van Moorsel}, {Coline Hm} and {van der Ent}, {Cornelis K} and Edwin Cuppen and {van Oudenaarden}, Alexander and Coenjaerts, {Frank E} and Linde Meyaard and Bont, {Louis J} and Peters, {Peter J} and Tans, {Sander J} and {van Zon}, {Jeroen S} and Boj, {Sylvia F} and Vries, {Robert G} and Beekman, {Jeffrey M} and Hans Clevers",

note = "{\textcopyright} 2019 The Authors. Published under the terms of the CC BY NC ND 4.0 license.",

year = "2019",

month = feb,

day = "15",

doi = "10.15252/embj.2018100300",

language = "English",

volume = "38",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Nature Publishing Group",

number = "4",

}

Sachs, N, Papaspyropoulos, A, Zomer-van Ommen, DD, Heo, I, Böttinger, L, Klay, D, Weeber, F, Huelsz-Prince, G, Iakobachvili, N, Amatngalim, GD, de Ligt, J, van Hoeck, A, Proost, N, Viveen, MC, Lyubimova, A, Teeven, L, Derakhshan, S, Korving, J, Begthel, H, Dekkers, JF, Kumawat, K, Ramos, E, van Oosterhout, MF, Offerhaus, GJ, Wiener, DJ, Olimpio, EP, Dijkstra, KK, Smit, EF, van der Linden, M, Jaksani, S, van de Ven, M, Jonkers, J, Rios, AC, Voest, EE, van Moorsel, CH, van der Ent, CK, Cuppen, E, van Oudenaarden, A, Coenjaerts, FE, Meyaard, L, Bont, LJ, Peters, PJ, Tans, SJ, van Zon, JS, Boj, SF, Vries, RG, Beekman, JM & Clevers, H 2019, 'Long-term expanding human airway organoids for disease modeling', EMBO Journal, vol. 38, nr. 4. https://doi.org/10.15252/embj.2018100300

TY - JOUR

T1 - Long-term expanding human airway organoids for disease modeling

AU - Sachs, Norman

AU - Papaspyropoulos, Angelos

AU - Zomer-van Ommen, Domenique D

AU - Heo, Inha

AU - Böttinger, Lena

AU - Klay, Dymph

AU - Weeber, Fleur

AU - Huelsz-Prince, Guizela

AU - Iakobachvili, Nino

AU - Amatngalim, Gimano D

AU - de Ligt, Joep

AU - van Hoeck, Arne

AU - Proost, Natalie

AU - Viveen, Marco C

AU - Lyubimova, Anna

AU - Teeven, Luc

AU - Derakhshan, Sepideh

AU - Korving, Jeroen

AU - Begthel, Harry

AU - Dekkers, Johanna F

AU - Kumawat, Kuldeep

AU - Ramos, Emilio

AU - van Oosterhout, Matthijs Fm

AU - Offerhaus, G Johan

AU - Wiener, Dominique J

AU - Olimpio, Eduardo P

AU - Dijkstra, Krijn K

AU - Smit, Egbert F

AU - van der Linden, Maarten

AU - Jaksani, Sridevi

AU - van de Ven, Marieke

AU - Jonkers, Jos

AU - Rios, Anne C

AU - Voest, Emile E

AU - van Moorsel, Coline Hm

AU - van der Ent, Cornelis K

AU - Cuppen, Edwin

AU - van Oudenaarden, Alexander

AU - Coenjaerts, Frank E

AU - Meyaard, Linde

AU - Bont, Louis J

AU - Peters, Peter J

AU - Tans, Sander J

AU - van Zon, Jeroen S

AU - Boj, Sylvia F

AU - Vries, Robert G

AU - Beekman, Jeffrey M

AU - Clevers, Hans

PY - 2019/2/15

Y1 - 2019/2/15

N2 - Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease.

AB - Organoids are self-organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long-term-expanding human airway organoids from broncho-alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi-ciliated cells, mucus-producing secretory cells, and CC10-secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR function in an organoid swelling assay. Organoids established from lung cancer resections and metastasis biopsies retain tumor histopathology as well as cancer gene mutations and are amenable to drug screening. Respiratory syncytial virus (RSV) infection recapitulates central disease features, dramatically increases organoid cell motility via the non-structural viral NS2 protein, and preferentially recruits neutrophils upon co-culturing. We conclude that human airway organoids represent versatile models for the in vitro study of hereditary, malignant, and infectious pulmonary disease.

U2 - 10.15252/embj.2018100300

DO - 10.15252/embj.2018100300

M3 - Article

C2 - 30643021

SN - 0261-4189

VL - 38

JO - EMBO Journal

JF - EMBO Journal

IS - 4

ER -

Long-term expanding human airway organoids for disease modeling

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