Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures

Kim E Boonekamp, Kai Kretzschmar, Dominique J Wiener, Priyanca Asra, Sepideh Derakhshan, Jens Puschhof, Carmen López-Iglesias, Peter J Peters, Onur Basak, Hans Clevers

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion media combines atypically high calcium concentrations, activation of cAMP, FGF, and R-spondin signaling with inhibition of bone morphogenetic protein (BMP) signaling. Organoids are established robustly from adult mouse skin and expand over at least 6 mo, while maintaining the basal-apical organization of the mouse interfollicular epidermis. The system represents a powerful tool to study epidermal homeostasis and disease in vitro.

Originele taal-2Engels
Pagina's (van-tot)14630-14638
Aantal pagina's9
TijdschriftProceedings of the National Academy of Sciences of the United States of America
Volume116
Nummer van het tijdschrift29
DOI's
StatusGepubliceerd - 16 jul 2019

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