Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures

Kim E Boonekamp; Kai Kretzschmar; Dominique J Wiener; Priyanca Asra; Sepideh Derakhshan; Jens Puschhof; Carmen López-Iglesias; Peter J Peters; Onur Basak; Hans Clevers

doi:10.1073/pnas.1715272116

Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures

Kim E Boonekamp, Kai Kretzschmar, Dominique J Wiener, Priyanca Asra, Sepideh Derakhshan, Jens Puschhof, Carmen López-Iglesias, Peter J Peters, Onur Basak, Hans Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

62 Citaten (Scopus)

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Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion media combines atypically high calcium concentrations, activation of cAMP, FGF, and R-spondin signaling with inhibition of bone morphogenetic protein (BMP) signaling. Organoids are established robustly from adult mouse skin and expand over at least 6 mo, while maintaining the basal-apical organization of the mouse interfollicular epidermis. The system represents a powerful tool to study epidermal homeostasis and disease in vitro.

Originele taal-2	Engels
Pagina's (van-tot)	14630-14638
Aantal pagina's	9
Tijdschrift	Proceedings of the National Academy of Sciences of the United States of America
Volume	116
Nummer van het tijdschrift	29
DOI's	https://doi.org/10.1073/pnas.1715272116
Status	Gepubliceerd - 16 jul. 2019

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10.1073/pnas.1715272116

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Boonekamp, K. E., Kretzschmar, K., Wiener, D. J., Asra, P., Derakhshan, S., Puschhof, J., López-Iglesias, C., Peters, P. J., Basak, O., & Clevers, H. (2019). Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures. Proceedings of the National Academy of Sciences of the United States of America, 116(29), 14630-14638. https://doi.org/10.1073/pnas.1715272116

@article{aa1175d2a7b54e8c9158473654fc8428,

title = "Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures",

abstract = "Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion media combines atypically high calcium concentrations, activation of cAMP, FGF, and R-spondin signaling with inhibition of bone morphogenetic protein (BMP) signaling. Organoids are established robustly from adult mouse skin and expand over at least 6 mo, while maintaining the basal-apical organization of the mouse interfollicular epidermis. The system represents a powerful tool to study epidermal homeostasis and disease in vitro.",

author = "Boonekamp, {Kim E} and Kai Kretzschmar and Wiener, {Dominique J} and Priyanca Asra and Sepideh Derakhshan and Jens Puschhof and Carmen L{\'o}pez-Iglesias and Peters, {Peter J} and Onur Basak and Hans Clevers",

year = "2019",

month = jul,

day = "16",

doi = "10.1073/pnas.1715272116",

language = "English",

volume = "116",

pages = "14630--14638",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Boonekamp, KE, Kretzschmar, K, Wiener, DJ, Asra, P, Derakhshan, S, Puschhof, J, López-Iglesias, C, Peters, PJ, Basak, O & Clevers, H 2019, 'Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, nr. 29, blz. 14630-14638. https://doi.org/10.1073/pnas.1715272116

Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures. / Boonekamp, Kim E; Kretzschmar, Kai; Wiener, Dominique J et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, Nr. 29, 16.07.2019, blz. 14630-14638.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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T1 - Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures

AU - Boonekamp, Kim E

AU - Kretzschmar, Kai

AU - Wiener, Dominique J

AU - Asra, Priyanca

AU - Derakhshan, Sepideh

AU - Puschhof, Jens

AU - López-Iglesias, Carmen

AU - Peters, Peter J

AU - Basak, Onur

AU - Clevers, Hans

PY - 2019/7/16

Y1 - 2019/7/16

N2 - Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion media combines atypically high calcium concentrations, activation of cAMP, FGF, and R-spondin signaling with inhibition of bone morphogenetic protein (BMP) signaling. Organoids are established robustly from adult mouse skin and expand over at least 6 mo, while maintaining the basal-apical organization of the mouse interfollicular epidermis. The system represents a powerful tool to study epidermal homeostasis and disease in vitro.

AB - Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an epidermal organoid culture system that allows long-term, genetically stable expansion of adult epidermal stem cells. Our epidermal expansion media combines atypically high calcium concentrations, activation of cAMP, FGF, and R-spondin signaling with inhibition of bone morphogenetic protein (BMP) signaling. Organoids are established robustly from adult mouse skin and expand over at least 6 mo, while maintaining the basal-apical organization of the mouse interfollicular epidermis. The system represents a powerful tool to study epidermal homeostasis and disease in vitro.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures

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