Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors

Daisong Wang, Jingqiang Wang, Lanyue Bai, Hong Pan, Hua Feng, Hans Clevers, Yi Arial Zeng

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

It has generally proven challenging to produce functional β cells in vitro. Here, we describe a previously unidentified protein C receptor positive (Procr+) cell population in adult mouse pancreas through single-cell RNA sequencing (scRNA-seq). The cells reside in islets, do not express differentiation markers, and feature epithelial-to-mesenchymal transition characteristics. By genetic lineage tracing, Procr+ islet cells undergo clonal expansion and generate all four endocrine cell types during adult homeostasis. Sorted Procr+ cells, representing ∼1% of islet cells, can robustly form islet-like organoids when cultured at clonal density. Exponential expansion can be maintained over long periods by serial passaging, while differentiation can be induced at any time point in culture. β cells dominate in differentiated islet organoids, while α, δ, and PP cells occur at lower frequencies. The organoids are glucose-responsive and insulin-secreting. Upon transplantation in diabetic mice, these organoids reverse disease. These findings demonstrate that the adult mouse pancreatic islet contains a population of Procr+ endocrine progenitors.

Originele taal-2Engels
Pagina's (van-tot)1198-1211.e19
TijdschriftCell
Volume180
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - 19 mrt 2020

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    Wang, D., Wang, J., Bai, L., Pan, H., Feng, H., Clevers, H., & Zeng, Y. A. (2020). Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors. Cell, 180(6), 1198-1211.e19. https://doi.org/10.1016/j.cell.2020.02.048