Low-cost, rapid and reliable DNA extraction from mammalian organoids using a C. elegans toolbox

Jonas Mars; Gabriella S Darmasaputra; Matilde Galli; Hendrik C Korswagen

doi:10.17912/micropub.biology.001240

Low-cost, rapid and reliable DNA extraction from mammalian organoids using a C. elegans toolbox

Jonas Mars, Gabriella S Darmasaputra, Matilde Galli, Hendrik C Korswagen

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

Samenvatting

High-quality DNA extraction from organoids is an important step in molecular genetics research. Here, we show that a lysis buffer from the field of Caenorhabditis elegans research, called Single Worm Lysis Buffer (SWLB), is a low-cost, yet reliable method for DNA extraction from mammalian organoids. SWLB is superior in terms of price, storage, hands-on time and sustainability compared to current standardized DNA extraction protocols, while equally effective. This work indicates that it is useful to compare methods from different model systems, such as mammalian organoids and invertebrate nematodes, to find useful alternatives for research methodologies.

Originele taal-2	Engels
Tijdschrift	microPublication biology
Volume	2024
DOI's	https://doi.org/10.17912/micropub.biology.001240
Status	Gepubliceerd - 2024

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10.17912/micropub.biology.001240

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