Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

E. Berezikov, G. van Tetering, M. Verheul, J. van de Belt, L.W. van Laake, J. Vos, R. Verloop, M.L. van de Wetering, V. Guryev, S. Takada, A.J. van Zonneveld, H. Mano, R. Plasterk, E. Cuppen

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently > 400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.
Originele taal-2Engels
Pagina's (van-tot)1289-1298
Aantal pagina's10
TijdschriftGenome Research
Volume16
Nummer van het tijdschrift10
DOI's
StatusGepubliceerd - 2006

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