Mechanism of antibacterial activity of the white-rot fungus Hypholoma fasciculare colonizing wood

TY - JOUR

T1 - Mechanism of antibacterial activity of the white-rot fungus Hypholoma fasciculare colonizing wood

AU - De Boer, W.

AU - Folman, L.B.

AU - Klein Gunnewiek, P.J.A.

AU - Svensson, T.

AU - Bastviken, D.

AU - Oberg, G.

AU - Del Rio, J.C.

AU - Boddy, L.

N1 - Reporting year: 2010 Metis note: 4694;CTE; ME; file:///L:\EndnoteDatabases\NIOOPUB\pdfs\PDFS2010\DeBoer_ea_4694.pdf

PY - 2010

Y1 - 2010

N2 - In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activity. Hypholoma fasciculare was allowed to invade beech and pine (Pinus sylvestris) wood blocks that had been precolonized by microorganisms from forest soil. The changes in the number of bacteria, fungal biomass, and fungal-related wood properties were followed for 23 weeks. Colonization by the fungus resulted in a rapid and large reduction in the number of bacteria (colony-forming units), which was already apparent after 4 weeks of incubation. The reduction in the number of bacteria coincided with fungal-induced acidification in both beech and pine wood blocks. No evidence was found for the involvement of toxic secondary metabolites or reactive oxygen species in the reduction of the number of bacteria. Additional experiments showed that the dominant bacteria present in the wood blocks were not able to grow under the acidic conditions (pH 3.5) created by the fungus. Hence our research pointed at rapid acidification as the major factor causing reduction of wood-inhabiting bacteria upon colonization of wood by H. fasciculare.

AB - In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activity. Hypholoma fasciculare was allowed to invade beech and pine (Pinus sylvestris) wood blocks that had been precolonized by microorganisms from forest soil. The changes in the number of bacteria, fungal biomass, and fungal-related wood properties were followed for 23 weeks. Colonization by the fungus resulted in a rapid and large reduction in the number of bacteria (colony-forming units), which was already apparent after 4 weeks of incubation. The reduction in the number of bacteria coincided with fungal-induced acidification in both beech and pine wood blocks. No evidence was found for the involvement of toxic secondary metabolites or reactive oxygen species in the reduction of the number of bacteria. Additional experiments showed that the dominant bacteria present in the wood blocks were not able to grow under the acidic conditions (pH 3.5) created by the fungus. Hence our research pointed at rapid acidification as the major factor causing reduction of wood-inhabiting bacteria upon colonization of wood by H. fasciculare.

U2 - 10.1139/W10-023

DO - 10.1139/W10-023

M3 - Article

SN - 0008-4166

VL - 56

SP - 380

EP - 388

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

IS - 5

ER -