TY - JOUR
T1 - Methane-derived carbon flows through methane-oxidizing bacteria to higher trophic levels in aquatic systems
AU - Deines, P.
AU - Bodelier, P.L.E.
AU - Eller, G.
N1 - Reporting year: 2007
Metis note: 3970;CL; MWE; file:///C:/pdfs/Pdfs2007/Deines_ea_3970.pdf
PY - 2007
Y1 - 2007
N2 - Recent investigations have shown that biogenic methane can be a carbon source for macro invertebrates in freshwater food webs. Stable carbon isotopic signatures, used to infer an organism's food source, indicated that methane can play a major role in the nutrition of chironomid larvae. However, the pathway of methane-derived carbon into invertebrate biomass is still not confirmed. It has been proposed that chironomid larvae ingest methane-oxidizing bacteria (MOB), but this has not been experimentally demonstrated to date. Using 13C-labelled methane we could show for the first time that chironomid larvae assimilate methane-derived carbon through MOB. Chironomid larval biomass was significantly 13C-enriched after dwelling for 10 days in lake sediment enriched with labelled methane. Moreover, phospholipid fatty acids diagnostic for MOB were detected in larval tissue and were significantly 13C-enriched, which encompasses the 13C-uptake predicted for a methane-based nutrition. Additionally, chironomid larvae fed on sediment and water-column derived MOB biomass.
AB - Recent investigations have shown that biogenic methane can be a carbon source for macro invertebrates in freshwater food webs. Stable carbon isotopic signatures, used to infer an organism's food source, indicated that methane can play a major role in the nutrition of chironomid larvae. However, the pathway of methane-derived carbon into invertebrate biomass is still not confirmed. It has been proposed that chironomid larvae ingest methane-oxidizing bacteria (MOB), but this has not been experimentally demonstrated to date. Using 13C-labelled methane we could show for the first time that chironomid larvae assimilate methane-derived carbon through MOB. Chironomid larval biomass was significantly 13C-enriched after dwelling for 10 days in lake sediment enriched with labelled methane. Moreover, phospholipid fatty acids diagnostic for MOB were detected in larval tissue and were significantly 13C-enriched, which encompasses the 13C-uptake predicted for a methane-based nutrition. Additionally, chironomid larvae fed on sediment and water-column derived MOB biomass.
U2 - 10.1111/j.1462-2920.2006.01235.x
DO - 10.1111/j.1462-2920.2006.01235.x
M3 - Article
SN - 1462-2912
VL - 9
SP - 1126
EP - 1134
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 5
ER -