TY - JOUR
T1 - Microbial secondary succession in a chronosequence of chalk grasslands
AU - Kuramae, E.E.
AU - Gamper, H.A.
AU - Yergeau, E.
AU - Piceno, Y.M.
AU - Brodie, E.L.
AU - DeSantis, T.Z.
AU - Andersen, G.L.
AU - Van Veen, J.A.
AU - Kowalchuk, G.A.
N1 - Reporting year: 2010
Metis note: 4732;CTE; ME; file:///L:\EndnoteDatabases\NIOOPUB\pdfs\PDFS2010\Kuramae_ea_4732.pdf
PY - 2010
Y1 - 2010
N2 - Although secondary succession has been studied extensively, we have little knowledge of the succession of soil-borne microbial communities. In this study, we therefore examined the structures of the microbial communities across two separate chronosequences of chalk grasslands in Limburg, the Netherlands, which are at different stages of secondary succession after being abandoned for between 17 and >66 years. Arable fields were also included in the investigation as non-abandoned references. Changes in the soil-borne microbial communities, as determined by phylogenetic microarray and quantitative PCR methodologies, were correlated with the prevailing environmental conditions related to vegetation and soil biochemistry. We observed clear patterns of microbial secondary succession related to soil age, pH and phosphate status, as exemplified by the overrepresentation of Verrucomicrobia, Acidobacteria, Gemmatimonadetes, and α-, δ- and ε-Proteobacteria at late successional stages. Moreover, effects of secondary succession versus changes in soil pH could be resolved, with pH significantly altering the trajectory of microbial succession.
AB - Although secondary succession has been studied extensively, we have little knowledge of the succession of soil-borne microbial communities. In this study, we therefore examined the structures of the microbial communities across two separate chronosequences of chalk grasslands in Limburg, the Netherlands, which are at different stages of secondary succession after being abandoned for between 17 and >66 years. Arable fields were also included in the investigation as non-abandoned references. Changes in the soil-borne microbial communities, as determined by phylogenetic microarray and quantitative PCR methodologies, were correlated with the prevailing environmental conditions related to vegetation and soil biochemistry. We observed clear patterns of microbial secondary succession related to soil age, pH and phosphate status, as exemplified by the overrepresentation of Verrucomicrobia, Acidobacteria, Gemmatimonadetes, and α-, δ- and ε-Proteobacteria at late successional stages. Moreover, effects of secondary succession versus changes in soil pH could be resolved, with pH significantly altering the trajectory of microbial succession.
U2 - 10.1038/ismej.2010.11
DO - 10.1038/ismej.2010.11
M3 - Article
SN - 1751-7362
VL - 4
SP - 711
EP - 715
JO - ISME Journal
JF - ISME Journal
IS - 5
ER -