Modelling Cryptosporidium infection in human small intestinal and lung organoids

Inha Heo; Devanjali Dutta; Deborah A Schaefer; Nino Iakobachvili; Benedetta Artegiani; Norman Sachs; Kim E Boonekamp; Gregory Bowden; Antoni P A Hendrickx; Robert J L Willems; Peter J Peters; Michael W Riggs; Roberta O'Connor; Hans Clevers

doi:10.1038/s41564-018-0177-8

Modelling Cryptosporidium infection in human small intestinal and lung organoids

Inha Heo, Devanjali Dutta, Deborah A Schaefer, Nino Iakobachvili, Benedetta Artegiani, Norman Sachs, Kim E Boonekamp, Gregory Bowden, Antoni P A Hendrickx, Robert J L Willems, Peter J Peters, Michael W Riggs, Roberta O'Connor, Hans Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

291 Citaten (Scopus)

Samenvatting

Stem-cell-derived organoids recapitulate in vivo physiology of their original tissues, representing valuable systems to model medical disorders such as infectious diseases. Cryptosporidium, a protozoan parasite, is a leading cause of diarrhoea and a major cause of child mortality worldwide. Drug development requires detailed knowledge of the pathophysiology of Cryptosporidium, but experimental approaches have been hindered by the lack of an optimal in vitro culture system. Here, we show that Cryptosporidium can infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. Temporal analysis of the Cryptosporidium transcriptome during organoid infection reveals dynamic regulation of transcripts related to its life cycle. Our study presents organoids as a physiologically relevant in vitro model system to study Cryptosporidium infection.

Originele taal-2	Engels
Pagina's (van-tot)	814-823
Aantal pagina's	10
Tijdschrift	Nature Microbiology
Volume	3
Nummer van het tijdschrift	7
DOI's	https://doi.org/10.1038/s41564-018-0177-8
Status	Gepubliceerd - jul. 2018

Toegang tot document

10.1038/s41564-018-0177-8

Citeer dit

Heo, I., Dutta, D., Schaefer, D. A., Iakobachvili, N., Artegiani, B., Sachs, N., Boonekamp, K. E., Bowden, G., Hendrickx, A. P. A., Willems, R. J. L., Peters, P. J., Riggs, M. W., O'Connor, R., & Clevers, H. (2018). Modelling Cryptosporidium infection in human small intestinal and lung organoids. Nature Microbiology, 3(7), 814-823. https://doi.org/10.1038/s41564-018-0177-8

@article{53b940b7051e42d0bdc5c7296d91aa3f,

title = "Modelling Cryptosporidium infection in human small intestinal and lung organoids",

abstract = "Stem-cell-derived organoids recapitulate in vivo physiology of their original tissues, representing valuable systems to model medical disorders such as infectious diseases. Cryptosporidium, a protozoan parasite, is a leading cause of diarrhoea and a major cause of child mortality worldwide. Drug development requires detailed knowledge of the pathophysiology of Cryptosporidium, but experimental approaches have been hindered by the lack of an optimal in vitro culture system. Here, we show that Cryptosporidium can infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. Temporal analysis of the Cryptosporidium transcriptome during organoid infection reveals dynamic regulation of transcripts related to its life cycle. Our study presents organoids as a physiologically relevant in vitro model system to study Cryptosporidium infection.",

author = "Inha Heo and Devanjali Dutta and Schaefer, {Deborah A} and Nino Iakobachvili and Benedetta Artegiani and Norman Sachs and Boonekamp, {Kim E} and Gregory Bowden and Hendrickx, {Antoni P A} and Willems, {Robert J L} and Peters, {Peter J} and Riggs, {Michael W} and Roberta O'Connor and Hans Clevers",

year = "2018",

month = jul,

doi = "10.1038/s41564-018-0177-8",

language = "English",

volume = "3",

pages = "814--823",

journal = "Nature Microbiology",

issn = "2058-5276",

publisher = "Nature Publishing Group",

number = "7",

}

TY - JOUR

T1 - Modelling Cryptosporidium infection in human small intestinal and lung organoids

AU - Heo, Inha

AU - Dutta, Devanjali

AU - Schaefer, Deborah A

AU - Iakobachvili, Nino

AU - Artegiani, Benedetta

AU - Sachs, Norman

AU - Boonekamp, Kim E

AU - Bowden, Gregory

AU - Hendrickx, Antoni P A

AU - Willems, Robert J L

AU - Peters, Peter J

AU - Riggs, Michael W

AU - O'Connor, Roberta

AU - Clevers, Hans

PY - 2018/7

Y1 - 2018/7

N2 - Stem-cell-derived organoids recapitulate in vivo physiology of their original tissues, representing valuable systems to model medical disorders such as infectious diseases. Cryptosporidium, a protozoan parasite, is a leading cause of diarrhoea and a major cause of child mortality worldwide. Drug development requires detailed knowledge of the pathophysiology of Cryptosporidium, but experimental approaches have been hindered by the lack of an optimal in vitro culture system. Here, we show that Cryptosporidium can infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. Temporal analysis of the Cryptosporidium transcriptome during organoid infection reveals dynamic regulation of transcripts related to its life cycle. Our study presents organoids as a physiologically relevant in vitro model system to study Cryptosporidium infection.

AB - Stem-cell-derived organoids recapitulate in vivo physiology of their original tissues, representing valuable systems to model medical disorders such as infectious diseases. Cryptosporidium, a protozoan parasite, is a leading cause of diarrhoea and a major cause of child mortality worldwide. Drug development requires detailed knowledge of the pathophysiology of Cryptosporidium, but experimental approaches have been hindered by the lack of an optimal in vitro culture system. Here, we show that Cryptosporidium can infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. Temporal analysis of the Cryptosporidium transcriptome during organoid infection reveals dynamic regulation of transcripts related to its life cycle. Our study presents organoids as a physiologically relevant in vitro model system to study Cryptosporidium infection.

U2 - 10.1038/s41564-018-0177-8

DO - 10.1038/s41564-018-0177-8

M3 - Article

C2 - 29946163

SN - 2058-5276

VL - 3

SP - 814

EP - 823

JO - Nature Microbiology

JF - Nature Microbiology

IS - 7

ER -

Modelling Cryptosporidium infection in human small intestinal and lung organoids

Samenvatting

Toegang tot document

Vingerafdruk

Citeer dit