Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru: Revista Iberoamericana De Micologia

V. Bejar; M. Tello; R. Garcia; J. M. Guevara; S. Gonzales; G. Vergaray; E. Valencia; E. Abanto; A. G. Ortega-Loayza; F. Hagen; E. L. Gutierrez

doi:10.1016/j.riam.2014.01.005

Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru: Revista Iberoamericana De Micologia

V. Bejar, M. Tello, R. Garcia, J. M. Guevara, S. Gonzales, G. Vergaray, E. Valencia, E. Abanto, A. G. Ortega-Loayza, F. Hagen, E. L. Gutierrez

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10 Citaten (Scopus)

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Background: Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii. Aims: To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neoformans strains from Lima, Peru. Methods: A set of 32 Cryptococcus spp. strains from the Institute of Tropical Medicine of the National University of San Marcos, Lima, Peru, were included in this retrospective study. Twenty-four strains were isolated from patients, while the remaining 8 were isolated from the environment. Results: Using conventional PCR, 27 (84.4%) of the isolates were identified as C neoformans var. grubii mating-type alpha and serotype A. Using the AFLP fingerprinting, it was shown that 16 (50%) of the C. neoformans strains were genotype AFLP1, 13 (40.6%) were genotype AFLP1B, 2 (63%) were genotype AFLP2, and 1 (3.1%) was found to be a hybrid between both C. neoformans varieties (genotype AFLP3). The antifungal susceptibility profiles for amphotericin B, fluconazole and voriconazole showed that all the 32 C neoformans are sensitive to these antifungal compounds. Conclusions: In this study we observed that C neoformans var. grubii (AFLP1 and AFLP1B) and C neoformans var. neoformans (AFLP2) were the only cryptococcal varieties involved. All strains were found to be sensitive to the antifungals tested, results that are consistent with those found in the international literature. (C) 2013 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved.

Originele taal-2	Engels
Pagina's (van-tot)	88-92
Aantal pagina's	5
Tijdschrift	Revista Iberoamericana de Micología
Volume	32
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/j.riam.2014.01.005
Status	Gepubliceerd - 2015

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10.1016/j.riam.2014.01.005

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Bejar, V., Tello, M., Garcia, R., Guevara, J. M., Gonzales, S., Vergaray, G., Valencia, E., Abanto, E., Ortega-Loayza, A. G., Hagen, F., & Gutierrez, E. L. (2015). Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru: Revista Iberoamericana De Micologia. Revista Iberoamericana de Micología, 32(2), 88-92. https://doi.org/10.1016/j.riam.2014.01.005

@article{280d2a5f8ead4244998bb8721d5374a2,

title = "Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru: Revista Iberoamericana De Micologia",

abstract = "Background: Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii. Aims: To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neoformans strains from Lima, Peru. Methods: A set of 32 Cryptococcus spp. strains from the Institute of Tropical Medicine of the National University of San Marcos, Lima, Peru, were included in this retrospective study. Twenty-four strains were isolated from patients, while the remaining 8 were isolated from the environment. Results: Using conventional PCR, 27 (84.4%) of the isolates were identified as C neoformans var. grubii mating-type alpha and serotype A. Using the AFLP fingerprinting, it was shown that 16 (50%) of the C. neoformans strains were genotype AFLP1, 13 (40.6%) were genotype AFLP1B, 2 (63%) were genotype AFLP2, and 1 (3.1%) was found to be a hybrid between both C. neoformans varieties (genotype AFLP3). The antifungal susceptibility profiles for amphotericin B, fluconazole and voriconazole showed that all the 32 C neoformans are sensitive to these antifungal compounds. Conclusions: In this study we observed that C neoformans var. grubii (AFLP1 and AFLP1B) and C neoformans var. neoformans (AFLP2) were the only cryptococcal varieties involved. All strains were found to be sensitive to the antifungals tested, results that are consistent with those found in the international literature. (C) 2013 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved.",

keywords = "Cryptococcus neoformans Mating-type Serotyping Genotype aids patients fluconazole diversity serotype brazil",

author = "V. Bejar and M. Tello and R. Garcia and Guevara, {J. M.} and S. Gonzales and G. Vergaray and E. Valencia and E. Abanto and Ortega-Loayza, {A. G.} and F. Hagen and Gutierrez, {E. L.}",

note = "ISI Document Delivery No.: CI8ZE Times Cited: 1 Cited Reference Count: 30 Bejar, Vilma Tello, Mercedes Garcia, Ruth Guevara, Jose M. Gonzales, Sofia Vergaray, German Valencia, Esther Abanto, Enma Ortega-Loayza, Alex G. Hagen, Ferry Gutierrez, Ericson L. Consejo Nacional de Ciencia y Tecnologia (CONCYTEC-Peru); Belgian Development Cooperation CTB-Peru [L11PER016] Mercedes Tello is supported by a scholarship for postgraduate studies in Cellular and Molecular Biology, from the Consejo Nacional de Ciencia y Tecnologia (CONCYTEC-Peru). Ericson Gutierrez is supported by a scholarship for postgraduate studies in Demography and Population, from the Belgian Development Cooperation CTB-Peru (L11PER016). Asociacion espanola micologia-aem Bilbao",

year = "2015",

doi = "10.1016/j.riam.2014.01.005",

language = "English",

volume = "32",

pages = "88--92",

journal = "Revista Iberoamericana de Micolog{\'i}a",

issn = "1130-1406",

publisher = "Asociacion Espanola de Micologia",

number = "2",

}

Bejar, V, Tello, M, Garcia, R, Guevara, JM, Gonzales, S, Vergaray, G, Valencia, E, Abanto, E, Ortega-Loayza, AG, Hagen, F & Gutierrez, EL 2015, 'Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru: Revista Iberoamericana De Micologia', Revista Iberoamericana de Micología, vol. 32, nr. 2, blz. 88-92. https://doi.org/10.1016/j.riam.2014.01.005

Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru: Revista Iberoamericana De Micologia. / Bejar, V.; Tello, M.; Garcia, R. et al.
In: Revista Iberoamericana de Micología, Vol. 32, Nr. 2, 2015, blz. 88-92.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru

T2 - Revista Iberoamericana De Micologia

AU - Bejar, V.

AU - Tello, M.

AU - Garcia, R.

AU - Guevara, J. M.

AU - Gonzales, S.

AU - Vergaray, G.

AU - Valencia, E.

AU - Abanto, E.

AU - Ortega-Loayza, A. G.

AU - Hagen, F.

AU - Gutierrez, E. L.

N1 - ISI Document Delivery No.: CI8ZE Times Cited: 1 Cited Reference Count: 30 Bejar, Vilma Tello, Mercedes Garcia, Ruth Guevara, Jose M. Gonzales, Sofia Vergaray, German Valencia, Esther Abanto, Enma Ortega-Loayza, Alex G. Hagen, Ferry Gutierrez, Ericson L. Consejo Nacional de Ciencia y Tecnologia (CONCYTEC-Peru); Belgian Development Cooperation CTB-Peru [L11PER016] Mercedes Tello is supported by a scholarship for postgraduate studies in Cellular and Molecular Biology, from the Consejo Nacional de Ciencia y Tecnologia (CONCYTEC-Peru). Ericson Gutierrez is supported by a scholarship for postgraduate studies in Demography and Population, from the Belgian Development Cooperation CTB-Peru (L11PER016). Asociacion espanola micologia-aem Bilbao

PY - 2015

Y1 - 2015

N2 - Background: Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii. Aims: To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neoformans strains from Lima, Peru. Methods: A set of 32 Cryptococcus spp. strains from the Institute of Tropical Medicine of the National University of San Marcos, Lima, Peru, were included in this retrospective study. Twenty-four strains were isolated from patients, while the remaining 8 were isolated from the environment. Results: Using conventional PCR, 27 (84.4%) of the isolates were identified as C neoformans var. grubii mating-type alpha and serotype A. Using the AFLP fingerprinting, it was shown that 16 (50%) of the C. neoformans strains were genotype AFLP1, 13 (40.6%) were genotype AFLP1B, 2 (63%) were genotype AFLP2, and 1 (3.1%) was found to be a hybrid between both C. neoformans varieties (genotype AFLP3). The antifungal susceptibility profiles for amphotericin B, fluconazole and voriconazole showed that all the 32 C neoformans are sensitive to these antifungal compounds. Conclusions: In this study we observed that C neoformans var. grubii (AFLP1 and AFLP1B) and C neoformans var. neoformans (AFLP2) were the only cryptococcal varieties involved. All strains were found to be sensitive to the antifungals tested, results that are consistent with those found in the international literature. (C) 2013 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved.

AB - Background: Cryptococcosis is a fungal infection with a worldwide distribution, mainly caused by Cryptococcus neoformans and Cryptococcus gattii. Aims: To molecularly characterize the mating-types, serotypes, genotypes and antifungal susceptibility profiles of a set of retrospectively isolated C. neoformans strains from Lima, Peru. Methods: A set of 32 Cryptococcus spp. strains from the Institute of Tropical Medicine of the National University of San Marcos, Lima, Peru, were included in this retrospective study. Twenty-four strains were isolated from patients, while the remaining 8 were isolated from the environment. Results: Using conventional PCR, 27 (84.4%) of the isolates were identified as C neoformans var. grubii mating-type alpha and serotype A. Using the AFLP fingerprinting, it was shown that 16 (50%) of the C. neoformans strains were genotype AFLP1, 13 (40.6%) were genotype AFLP1B, 2 (63%) were genotype AFLP2, and 1 (3.1%) was found to be a hybrid between both C. neoformans varieties (genotype AFLP3). The antifungal susceptibility profiles for amphotericin B, fluconazole and voriconazole showed that all the 32 C neoformans are sensitive to these antifungal compounds. Conclusions: In this study we observed that C neoformans var. grubii (AFLP1 and AFLP1B) and C neoformans var. neoformans (AFLP2) were the only cryptococcal varieties involved. All strains were found to be sensitive to the antifungals tested, results that are consistent with those found in the international literature. (C) 2013 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved.

KW - Cryptococcus neoformans Mating-type Serotyping Genotype aids patients fluconazole diversity serotype brazil

U2 - 10.1016/j.riam.2014.01.005

DO - 10.1016/j.riam.2014.01.005

M3 - Article

SN - 1130-1406

VL - 32

SP - 88

EP - 92

JO - Revista Iberoamericana de Micología

JF - Revista Iberoamericana de Micología

IS - 2

ER -

Molecular characterization and antifungal susceptibility of Cryptococcus neoformans strains collected from a single institution in Lima, Peru: Revista Iberoamericana De Micologia

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