Molecular engineering to improve lignocellulosic biomass based applications using filamentous fungi

TY - JOUR

T1 - Molecular engineering to improve lignocellulosic biomass based applications using filamentous fungi

AU - Meng, Jiali

AU - Mäkelä, Miia R

AU - de Vries, Ronald P

N1 - Copyright © 2021 Elsevier Inc. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Lignocellulosic biomass is an abundant and renewable resource, and its utilization has become the focus of research and biotechnology applications as a very promising raw material for the production of value-added compounds. Filamentous fungi play an important role in the production of various lignocellulolytic enzymes, while some of them have also been used for the production of important metabolites. However, wild type strains have limited efficiency in enzyme production or metabolic conversion, and therefore many efforts have been made to engineer improved strains. Examples of this are the manipulation of transcriptional regulators and/or promoters of enzyme-encoding genes to increase gene expression, and protein engineering to improve the biochemical characteristics of specific enzymes. This review provides and overview of the applications of filamentous fungi in lignocellulosic biomass based processes and the development and current status of various molecular engineering strategies to improve these processes.

AB - Lignocellulosic biomass is an abundant and renewable resource, and its utilization has become the focus of research and biotechnology applications as a very promising raw material for the production of value-added compounds. Filamentous fungi play an important role in the production of various lignocellulolytic enzymes, while some of them have also been used for the production of important metabolites. However, wild type strains have limited efficiency in enzyme production or metabolic conversion, and therefore many efforts have been made to engineer improved strains. Examples of this are the manipulation of transcriptional regulators and/or promoters of enzyme-encoding genes to increase gene expression, and protein engineering to improve the biochemical characteristics of specific enzymes. This review provides and overview of the applications of filamentous fungi in lignocellulosic biomass based processes and the development and current status of various molecular engineering strategies to improve these processes.

KW - Biomass

KW - Biotechnology

KW - Fungi/genetics

KW - Lignin

U2 - 10.1016/bs.aambs.2020.09.001

DO - 10.1016/bs.aambs.2020.09.001

M3 - Book/Film/Article review

C2 - 33934853

SN - 0065-2164

VL - 114

SP - 73

EP - 109

JO - Advances in Applied Microbiology

JF - Advances in Applied Microbiology

ER -