Molecular typing of Australian Scedosporium isolates showing genetic variability and numerous S. aurantiacum

Laurence Delhaes, Azian Harun, Sharon C A Chen, Quoc Nguyen, Monica Slavin, Christopher H Heath, Krystyna Maszewska, Catriona Halliday, Vincent Robert, Tania C Sorrell, Wieland Meyer,

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    One hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003-2005) and 46 additional isolates were genotyped by internal transcribed spacer-restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and PCR fingerprinting identified 3 distinct genetic groups. The first group corresponded to Scedosporium prolificans (n = 83), and the other 2 comprised isolates previously identified as S. apiospermum: one of these corresponded to S. apiospermum (n = 33) and the other to the newly described species S. aurantiacum (n = 30). Intraspecies variation was highest for S. apiospermum (58%), followed by S. prolificans (45%) and S. aurantiacum (28%) as determined by PCR fingerprinting. ITS sequence variation of 2.2% was observed among S. apiospermum isolates. No correlation was found between genotype of strains and their geographic origin, body site from which they were cultured, or colonization versus invasive disease. Twelve S. prolificans isolates from 2 suspected case clusters were examined by amplified fragment length polymorphism analysis. No specific clusters were confirmed.

    Originele taal-2Engels
    Pagina's (van-tot)282-290
    Aantal pagina's9
    TijdschriftEmerging Infectious Diseases
    Volume14
    Nummer van het tijdschrift2
    DOI's
    StatusGepubliceerd - feb. 2008

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