Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis

Vanessa Brito de Souza Rabello; Marcus de Melo Teixeira; Wieland Meyer; Laszlo Irinyi; Melissa Orzechowski Xavier; Vanice Rodrigues Poester; José Guillermo Pereira Brunelli; Fernando Almeida-Silva; Andrea Reis Bernardes-Engemann; Isabella Dib Ferreira Gremião; Débora Ferreira Dos Santos Angelo; Inácio José Clementino; Rodrigo Almeida-Paes; Rosely Maria Zancopé-Oliveira

doi:10.1016/j.fgb.2023.103845

Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis

Vanessa Brito de Souza Rabello, Marcus de Melo Teixeira, Wieland Meyer, Laszlo Irinyi, Melissa Orzechowski Xavier, Vanice Rodrigues Poester, José Guillermo Pereira Brunelli, Fernando Almeida-Silva, Andrea Reis Bernardes-Engemann, Isabella Dib Ferreira Gremião, Débora Ferreira Dos Santos Angelo, Inácio José Clementino, Rodrigo Almeida-Paes, Rosely Maria Zancopé-Oliveira

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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Sporotrichosis is a subcutaneous mycosis caused by pathogenic Sporothrix species. Among them, Sporothrix brasiliensis is the main species associated with endemic regions in South America, especially Brazil. It is highly virulent and can be spread through zoonotic transmission. Molecular epidemiological surveys are needed to determine the extent of genetic variation, to investigate outbreaks, and to identify genotypes associated with antifungal resistance and susceptibility. This study investigated the sequence variation of different constitutive genes and established a novel multilocus sequence typing (MLST) scheme for S. brasiliensis. Specific primers were designed for 16 genes using Primer-BLAST software based on the genome sequences of three S. brasiliensis strains (ATCC MYA-4823, A001 and A005). Ninety-one human, animal, and environmental S. brasiliensis isolates from different Brazilian geographic regions (South, Southeast, Midwest and Northeast) andtwo isolates from Paraguay were sequenced. The loci that presented the highest nucleotide diversity (π) were selected for the MLST scheme. Among the 16 studied genetic loci, four presented increased π value and were able to distinguish all S. brasiliensis isolates into seven distinct haplotypes. The PCR conditions were standardized for four loci. Some of the obtained haplotypes were associated with the geographic origin of the strains. This study presents an important advance in the understanding of this important agent of sporotrichosis in Brazil. It significantly increased the discriminatory power for genotyping of S. brasiliensis isolates, and enabled new contributions to the epidemiological studies of this human and animal pathogen in Brazil and in other countries.

Originele taal-2	Engels
Pagina's (van-tot)	103845
Tijdschrift	Fungal Genetics and Biology
Volume	170
DOI's	https://doi.org/10.1016/j.fgb.2023.103845
Status	Gepubliceerd - feb. 2024

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de Souza Rabello, V. B., de Melo Teixeira, M., Meyer, W., Irinyi, L., Xavier, M. O., Poester, V. R., Pereira Brunelli, J. G., Almeida-Silva, F., Bernardes-Engemann, A. R., Ferreira Gremião, I. D., Dos Santos Angelo, D. F., Clementino, I. J., Almeida-Paes, R., & Zancopé-Oliveira, R. M. (2024). Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis. Fungal Genetics and Biology, 170, 103845. https://doi.org/10.1016/j.fgb.2023.103845

@article{722db2bb3e474f44a30c999bb4cc5eea,

title = "Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis",

abstract = "Sporotrichosis is a subcutaneous mycosis caused by pathogenic Sporothrix species. Among them, Sporothrix brasiliensis is the main species associated with endemic regions in South America, especially Brazil. It is highly virulent and can be spread through zoonotic transmission. Molecular epidemiological surveys are needed to determine the extent of genetic variation, to investigate outbreaks, and to identify genotypes associated with antifungal resistance and susceptibility. This study investigated the sequence variation of different constitutive genes and established a novel multilocus sequence typing (MLST) scheme for S. brasiliensis. Specific primers were designed for 16 genes using Primer-BLAST software based on the genome sequences of three S. brasiliensis strains (ATCC MYA-4823, A001 and A005). Ninety-one human, animal, and environmental S. brasiliensis isolates from different Brazilian geographic regions (South, Southeast, Midwest and Northeast) andtwo isolates from Paraguay were sequenced. The loci that presented the highest nucleotide diversity (π) were selected for the MLST scheme. Among the 16 studied genetic loci, four presented increased π value and were able to distinguish all S. brasiliensis isolates into seven distinct haplotypes. The PCR conditions were standardized for four loci. Some of the obtained haplotypes were associated with the geographic origin of the strains. This study presents an important advance in the understanding of this important agent of sporotrichosis in Brazil. It significantly increased the discriminatory power for genotyping of S. brasiliensis isolates, and enabled new contributions to the epidemiological studies of this human and animal pathogen in Brazil and in other countries.",

author = "{de Souza Rabello}, {Vanessa Brito} and {de Melo Teixeira}, Marcus and Wieland Meyer and Laszlo Irinyi and Xavier, {Melissa Orzechowski} and Poester, {Vanice Rodrigues} and {Pereira Brunelli}, {Jos{\'e} Guillermo} and Fernando Almeida-Silva and Bernardes-Engemann, {Andrea Reis} and {Ferreira Gremi{\~a}o}, {Isabella Dib} and {Dos Santos Angelo}, {D{\'e}bora Ferreira} and Clementino, {In{\'a}cio Jos{\'e}} and Rodrigo Almeida-Paes and Zancop{\'e}-Oliveira, {Rosely Maria}",

year = "2024",

month = feb,

doi = "10.1016/j.fgb.2023.103845",

language = "English",

volume = "170",

pages = "103845",

journal = "Fungal Genetics and Biology",

issn = "1087-1845",

publisher = "Academic Press",

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de Souza Rabello, VB, de Melo Teixeira, M, Meyer, W, Irinyi, L, Xavier, MO, Poester, VR, Pereira Brunelli, JG, Almeida-Silva, F, Bernardes-Engemann, AR, Ferreira Gremião, ID, Dos Santos Angelo, DF, Clementino, IJ, Almeida-Paes, R & Zancopé-Oliveira, RM 2024, 'Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis', Fungal Genetics and Biology, vol. 170, blz. 103845. https://doi.org/10.1016/j.fgb.2023.103845

Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis. / de Souza Rabello, Vanessa Brito; de Melo Teixeira, Marcus; Meyer, Wieland et al.
In: Fungal Genetics and Biology, Vol. 170, 02.2024, blz. 103845.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis

AU - de Souza Rabello, Vanessa Brito

AU - de Melo Teixeira, Marcus

AU - Meyer, Wieland

AU - Irinyi, Laszlo

AU - Xavier, Melissa Orzechowski

AU - Poester, Vanice Rodrigues

AU - Pereira Brunelli, José Guillermo

AU - Almeida-Silva, Fernando

AU - Bernardes-Engemann, Andrea Reis

AU - Ferreira Gremião, Isabella Dib

AU - Dos Santos Angelo, Débora Ferreira

AU - Clementino, Inácio José

AU - Almeida-Paes, Rodrigo

AU - Zancopé-Oliveira, Rosely Maria

PY - 2024/2

Y1 - 2024/2

N2 - Sporotrichosis is a subcutaneous mycosis caused by pathogenic Sporothrix species. Among them, Sporothrix brasiliensis is the main species associated with endemic regions in South America, especially Brazil. It is highly virulent and can be spread through zoonotic transmission. Molecular epidemiological surveys are needed to determine the extent of genetic variation, to investigate outbreaks, and to identify genotypes associated with antifungal resistance and susceptibility. This study investigated the sequence variation of different constitutive genes and established a novel multilocus sequence typing (MLST) scheme for S. brasiliensis. Specific primers were designed for 16 genes using Primer-BLAST software based on the genome sequences of three S. brasiliensis strains (ATCC MYA-4823, A001 and A005). Ninety-one human, animal, and environmental S. brasiliensis isolates from different Brazilian geographic regions (South, Southeast, Midwest and Northeast) andtwo isolates from Paraguay were sequenced. The loci that presented the highest nucleotide diversity (π) were selected for the MLST scheme. Among the 16 studied genetic loci, four presented increased π value and were able to distinguish all S. brasiliensis isolates into seven distinct haplotypes. The PCR conditions were standardized for four loci. Some of the obtained haplotypes were associated with the geographic origin of the strains. This study presents an important advance in the understanding of this important agent of sporotrichosis in Brazil. It significantly increased the discriminatory power for genotyping of S. brasiliensis isolates, and enabled new contributions to the epidemiological studies of this human and animal pathogen in Brazil and in other countries.

AB - Sporotrichosis is a subcutaneous mycosis caused by pathogenic Sporothrix species. Among them, Sporothrix brasiliensis is the main species associated with endemic regions in South America, especially Brazil. It is highly virulent and can be spread through zoonotic transmission. Molecular epidemiological surveys are needed to determine the extent of genetic variation, to investigate outbreaks, and to identify genotypes associated with antifungal resistance and susceptibility. This study investigated the sequence variation of different constitutive genes and established a novel multilocus sequence typing (MLST) scheme for S. brasiliensis. Specific primers were designed for 16 genes using Primer-BLAST software based on the genome sequences of three S. brasiliensis strains (ATCC MYA-4823, A001 and A005). Ninety-one human, animal, and environmental S. brasiliensis isolates from different Brazilian geographic regions (South, Southeast, Midwest and Northeast) andtwo isolates from Paraguay were sequenced. The loci that presented the highest nucleotide diversity (π) were selected for the MLST scheme. Among the 16 studied genetic loci, four presented increased π value and were able to distinguish all S. brasiliensis isolates into seven distinct haplotypes. The PCR conditions were standardized for four loci. Some of the obtained haplotypes were associated with the geographic origin of the strains. This study presents an important advance in the understanding of this important agent of sporotrichosis in Brazil. It significantly increased the discriminatory power for genotyping of S. brasiliensis isolates, and enabled new contributions to the epidemiological studies of this human and animal pathogen in Brazil and in other countries.

U2 - 10.1016/j.fgb.2023.103845

DO - 10.1016/j.fgb.2023.103845

M3 - Article

C2 - 38040325

SN - 1087-1845

VL - 170

SP - 103845

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

ER -

Multi-locus sequencing typing reveals geographically related intraspecies variability of Sporothrix brasiliensis

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