NINscope, a versatile miniscope for multi-region circuit investigations

TY - JOUR

T1 - NINscope, a versatile miniscope for multi-region circuit investigations

AU - de Groot, Andres

AU - van den Boom, Bastijn Jg

AU - van Genderen, Romano M

AU - Coppens, Joris

AU - van Veldhuijzen, John

AU - Bos, Joop

AU - Hoedemaker, Hugo

AU - Negrello, Mario

AU - Willuhn, Ingo

AU - De Zeeuw, Chris I

AU - Hoogland, Tycho M

N1 - © 2020, de Groot et al.

PY - 2020/1/10

Y1 - 2020/1/10

N2 - Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform control software, our miniscope is a versatile addition to the expanding toolbox of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.

AB - Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform control software, our miniscope is a versatile addition to the expanding toolbox of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.

U2 - 10.7554/eLife.49987

DO - 10.7554/eLife.49987

M3 - Article

C2 - 31934857

SN - 2050-084X

VL - 9

JO - eLife

JF - eLife

M1 - e49987

ER -