Nitrosomonas europaea-like bacteria detected as the dominant b-subclass Proteobacteria ammonia oxidisers in reference and limed acid forest soils

TY - JOUR

T1 - Nitrosomonas europaea-like bacteria detected as the dominant b-subclass Proteobacteria ammonia oxidisers in reference and limed acid forest soils

AU - Carnol, M.

AU - Kowalchuk, G.A.

AU - De Boer, W.

N1 - Reporting year: 2002 Metis note: 3022; CTE; TME ; ME file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2002/Carnol_ea_3022.pdf

PY - 2002

Y1 - 2002

N2 - Net nitrification in intact soil cores and the community of ammonia-oxidising bacteria were studied in acid Norway spruce (Picea abies (L.) Karst) and sessile oak (Quercus petraea (Matt. Lieb.)) soils (Haute Ardenne, east Belgium) 18 months after treatment with 5tha1 dolomite lime. Liming caused a significant increase in nitrification in the upper soil layers (0.15m) of both stands. DGGE (denaturing gradient gel electrophoresis) profiling after -subclass ammonia oxidiser-specific polymerase chain reaction (PCR), combined with hybridisation and sequencing of excised DGGE bands revealed a dominance of Nitrosomonas europaea-like sequences, independent of soil horizon, tree species and lime treatment. A minority Nitrosospira-like population was detected, which showed affinity to nitrosospiras previously detected in acid soil. These results contrast with several reports suggesting a dominance of Nitrosospira-like organisms among ammonia oxidiser communities in acid soils. [KEYWORDS: 16S rDNA; DGGE; Picea abies; Quercus petraea; Nitrification; Dolomite lime]

AB - Net nitrification in intact soil cores and the community of ammonia-oxidising bacteria were studied in acid Norway spruce (Picea abies (L.) Karst) and sessile oak (Quercus petraea (Matt. Lieb.)) soils (Haute Ardenne, east Belgium) 18 months after treatment with 5tha1 dolomite lime. Liming caused a significant increase in nitrification in the upper soil layers (0.15m) of both stands. DGGE (denaturing gradient gel electrophoresis) profiling after -subclass ammonia oxidiser-specific polymerase chain reaction (PCR), combined with hybridisation and sequencing of excised DGGE bands revealed a dominance of Nitrosomonas europaea-like sequences, independent of soil horizon, tree species and lime treatment. A minority Nitrosospira-like population was detected, which showed affinity to nitrosospiras previously detected in acid soil. These results contrast with several reports suggesting a dominance of Nitrosospira-like organisms among ammonia oxidiser communities in acid soils. [KEYWORDS: 16S rDNA; DGGE; Picea abies; Quercus petraea; Nitrification; Dolomite lime]

U2 - 10.1016/S0038-0717(02)00039-1

DO - 10.1016/S0038-0717(02)00039-1

M3 - Article

SN - 0038-0717

VL - 34

SP - 1047

EP - 1050

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

IS - 7

ER -