Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture

TY - JOUR

T1 - Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture

AU - Tjalsma, Sjoerd Jd

AU - de Laat, Wouter

N1 - Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.

PY - 2020/11/18

Y1 - 2020/11/18

N2 - Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.

AB - Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.

U2 - 10.1016/j.gde.2020.10.002

DO - 10.1016/j.gde.2020.10.002

M3 - Book/Film/Article review

C2 - 33220512

SN - 0959-437X

VL - 67

SP - 10

EP - 17

JO - Current Opinion in Genetics and Development

JF - Current Opinion in Genetics and Development

ER -