TY - JOUR
T1 - Organoids and organs-on-chips
T2 - Insights into human gut-microbe interactions
AU - Puschhof, Jens
AU - Pleguezuelos-Manzano, Cayetano
AU - Clevers, Hans
N1 - Copyright © 2021 Elsevier Inc. All rights reserved.
PY - 2021/6/9
Y1 - 2021/6/9
N2 - The important and diverse roles of the gut microbiota in human health and disease are increasingly recognized. The difficulty of inferring causation from metagenomic microbiome sequencing studies and from mouse-human interspecies differences has prompted the development of sophisticated in vitro models of human gut-microbe interactions. Here, we review recent advances in the co-culture of microbes with intestinal and colonic epithelia, comparing the rapidly developing fields of organoids and organs-on-chips with other standard models. We describe how specific individual processes by which microbes and epithelia interact can be recapitulated in vitro. Using examples of bacterial, viral, and parasitic infections, we highlight the advantages of each culture model and discuss current trends and future possibilities to build more complex co-cultures.
AB - The important and diverse roles of the gut microbiota in human health and disease are increasingly recognized. The difficulty of inferring causation from metagenomic microbiome sequencing studies and from mouse-human interspecies differences has prompted the development of sophisticated in vitro models of human gut-microbe interactions. Here, we review recent advances in the co-culture of microbes with intestinal and colonic epithelia, comparing the rapidly developing fields of organoids and organs-on-chips with other standard models. We describe how specific individual processes by which microbes and epithelia interact can be recapitulated in vitro. Using examples of bacterial, viral, and parasitic infections, we highlight the advantages of each culture model and discuss current trends and future possibilities to build more complex co-cultures.
KW - Animal Testing Alternatives
KW - Animals
KW - Coculture Techniques/methods
KW - Gastrointestinal Microbiome
KW - Host Microbial Interactions
KW - Humans
KW - Intestinal Mucosa
KW - Mice
KW - Microbial Interactions
KW - Microchip Analytical Procedures/methods
KW - Microfluidics/methods
KW - Models, Animal
KW - Organoids/microbiology
KW - Precision Medicine/methods
U2 - 10.1016/j.chom.2021.04.002
DO - 10.1016/j.chom.2021.04.002
M3 - Book/Film/Article review
C2 - 34111395
SN - 1931-3128
VL - 29
SP - 867
EP - 878
JO - Cell Host & Microbe
JF - Cell Host & Microbe
IS - 6
ER -