Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology

Stefan Schulte-Merker, Didier Y R Stainier

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.

Originele taal-2Engels
Pagina's (van-tot)3103-4
Aantal pagina's2
TijdschriftDevelopment
Volume141
Nummer van het tijdschrift16
DOI's
StatusGepubliceerd - aug 2014

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